	   SnapGene      ,¢ACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCCTCGTTCATTCACGTTTTTGAACCCGTGGAGGACGGGCAGACTCGCGGTGCAAATGTGTTTTACAGCGTGATGGAGCAGATGAAGATGCTCGACACGCTGCAGAACACGCAGCTAGATTAACCCTAGAAAGATAATCATATTGTGACGTACGTTAAAGATAATCATGCGTAAAATTGACGCATGTGTTTTATCGGTCTGTATATCGAGGTTTATTTATTAATTTGAATAGATATTAAGTTTTATTATATTTACACTTACATACTAATAATAAATTCAACAAACAATTTATTTATGTTTATTTATTTATTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAACAAAACTTTTATGAGGGACAGCCCCCCCCCAAAGCCCCCAGGGATGTAATTACGTCCCTCCCCCGCTAGGGGGCAGCAGCGAGCCGCCCGGGGCTCCGCTCCGGTCCGGCGCTCCCCCCGCATCCCCGAGCCGGCAGCGTGCGGGGACAGCCCGGGCACGGGGAAGGTGGCACGGGATCGCTTTCCTCTGAACGCTTCTCGCTGCTCTTTGAGCCTGCAGACACCTGGGGGGATACGGGGAAAAGGCCTCCAAGGCCTACTAGTATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGATTCTAGAGCTAGCGAATTCGCCACCatgaaggcccccgctgtgcttgcacctggcatcctcgtgctcctgtttaccttggtgcagaggagcaatggggagtgtaaagaggcactagcaaagtccgagatgaatgtgaatatgaagtatcagcttcccaacttcaccgcggaaacacccatccagaatgtcattctacatgagcatcacattttccttggtgccactaactacatttatgttttaaatgaggaagaccttcagaaggttgctgagtacaagactgggcctgtgctggaacacccagattgtttcccatgtcaggactgcagcagcaaagccaatttatcaggaggtgtttggaaagataacatcaacatggctctagttgtcgacacctactatgatgatcaactcattagctgtggcagcgtcaacagagggacctgccagcgacatgtctttccccacaatcatactgctgacatacagtcggaggttcactgcatattctccccacagatagaagagcccagccagtgtcctgactgtgtggtgagcgccctgggagccaaagtcctttcatctgtaaaggaccggttcatcaacttctttgtaggcaataccataaattcttcttatttcccagatcatccattgcattcgatatcagtgagaaggctaaaggaaacgaaagatggttttatgtttttgacggaccagtcctacattgatgttttacctgagttcagagattcttaccccattaagtatgtccatgcctttgaaagcaacaattttatttacttcttgacggtccaaagggaaactctagatgctcagacttttcacacaagaataatcaggttctgttccataaactctggattgcattcctacatggaaatgcctctggagtgtattctcacagaaaagagaaaaaagagatccacaaagaaggaagtgtttaatatacttcaggctgcgtatgtcagcaagcctggggcccagcttgctagacaaataggagccagcctgaatgatgacattcttttcggggtgttcgcacaaagcaagccagattctgccgaaccaatggatcgatctgccatgtgtgcattccctatcaaatatgtcaacgacttcttcaacaagatcgtcaacaaaaacaatgtgagatgtctccagcatttttacggacccaatcatgagcactgctttaataggacacttctgagaaattcatcaggctgtgaagcgcgccgtgatgaatatcgaacagagtttaccacagctttgcagcgcgttgacttattcatgggtcaattcagcgaagtcctcttaacatctatatccaccttcattaaaggagacctcaccatagctaatcttgggacatcagagggtcgcttcatgcaggttgtggtttctcgatcaggaccatcaacccctcatgtgaattttctcctggactcccatccagtgtctccagaagtgattgtggagcatacattaaaccaaaatggctacacactggttatcactgggaagaagatcacgaagatcccattgaatggcttgggctgcagacatttccagtcctgcagtcaatgcctctctgccccaccctttgttcagtgtggctggtgccacgacaaatgtgtgcgatcggaggaatgcctgagcgggacatggactcaacagatctgtctgcctgcaatctacaaggttttcccaaatagtgcaccccttgaaggagggacaaggctgaccatatgtggctgggactttggatttcggaggaataataaatttgatttaaagaaaactagagttctccttggaaatgagagctgcaccttgactttaagtgagagcacgatgaatacattgaaatgcacagttggtcctgccatgaataagcatttcaatatgtccataattatttcaaatggccacgggacaacacaatacagtacattctcctatgtggatcctgtaataacaagtatttcgccgaaatacggtcctatggctggtggcactttacttactttaactggaaattacctaaacagtgggaattctagacacatttcaattggtggaaaaacatgtactttaaaaagtgtgtcaaacagtattcttgaatgttataccccagcccaaaccatttcaactgagtttgctgttaaattgaaaattgacttagccaaccgagagacaagcatcttcagttaccgtgaagatcccattgtctatgaaattcatccaaccaaatcttttattagtggtgggagcacaataacaggtgttgggaaaaacctgaattcagttagtgtcccgagaatggtcataaatgtgcatgaagcaggaaggaactttacagtggcatgtcaacatcgctctaattcagagataatctgttgtaccactccttccctgcaacagctgaatctgcaactccccctgaaaaccaaagcctttttcatgttagatgggatcctttccaaatactttgatctcatttatgtacataatcctgtgtttaagccttttgaaaagccagtgatgatctcaatgggcaatgaaaatgtactggaaattaagggaaatgatattgaccctgaagcagttaaaggtgaagtgttaaaagttggaaataagagctgtgagaatatacacttacattctgaagccgttttatgcacggtccccaatgacctgctgaaattgaacagcgagctaaatatagagtggaagcaagcaatttcttcaaccgtccttggaaaagtaatagttcaaccagatcagaatttcacaggattgattgctggtgttgtctcaatatcaacagcactgttattactacttgggtttttcctgtggctgaaaaagagaaagcaaattaaagatctgggcagtgaattagttcgctacgatgcaagagtacacactcctcatttggataggcttgtaagtgcccgaagtgtaagcccaactacagaaatggtttcaaatgaatctgtagactaccgagctacttttccagaagatcagtttcctaattcatctcagaacggttcatgccgacaagtgcagtatcctctgacagacatgtcccccatcctaactagtggggactctgatatatccagtccattactgcaaaatactgtccacattgacctcagtgctctaaatccagagctggtccaggcagtgcagcatgtagtgattgggcccagtagcctgattgtgcatttcaatgaagtcataggaagagggcattttggttgtgtatatcatgggactttgttggacaatgatggcaagaaaattcactgtgctgtgaaatccttgaacagaatcactgacataggagaagtttcccaatttctgaccgagggaatcatcatgaaagattttagtcatcccaatgtcctctcgctcctgggaatctgcctgcgaagtgaagggtctccgctggtggtcctaccatacatgaaacatggagatcttcgaaatttcattcgaaatgagactcataatccaactgtaaaagatcttattggctttggtcttcaagtagccaaaggcatgaaatatcttgcaagcaaaaagtttgtccacagagacttggctgcaagaaactgtatgctggatgaaaaattcacagtcaaggttgctgattttggtcttgccagaaacatgtatgataaagaatactatagtgtacacaacaaaacaggtgcaaagctgccagtgaagtggatggctttggaaagtctgcaaactcaaaagtttaccaccaagtcagatgtgtggtcctttggcgtgctcctctgggagctgatgacaagaggagccccaccttatcctgacgtaaacacctttgatataactgtttacttgttgcaagggagaagactcctacaacccgaatactgcccagaccccttatatgaagtaatgctaaaatgctggcaccctaaagccgaaatgcgcccatccttttctgaactggtgtcccggatatcagcgatcttctctactttcattggggagcactatgtccatgtgaacgctacttatgtgaacgtaaaatgtgtcgctccgtatccttctctgttgtcatcagaagataacgctgatgatgaggtggacacacgaccagcctccttctgggagacatcatagGATCCGCGGCCGCAAGGATCTGCGATCGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACGGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGCTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGAGGCCGCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCCTCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACCGGGCCTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTTTGCCTGACCCTGCTTGCTCAACTCTACGTCTTTGTTTCGTTTTCTGTTCTGCGCCGTTACAGATCCAAGCTGTGACCGGCGCCTACGCTAGACGCCACCATGGAGAGCGACGAGAGCGGCCTGCCCGCCATGGAGATCGAGTGCCGCATCACCGGCACCCTGAACGGCGTGGAGTTCGAGCTGGTGGGCGGCGGAGAGGGCACCCCCAAGCAGGGCCGCATGACCAACAAGATGAAGAGCACCAAAGGCGCCCTGACCTTCAGCCCCTACCTGCTGAGCCACGTGATGGGCTACGGCTTCTACCACTTCGGCACCTACCCCAGCGGCTACGAGAACCCCTTCCTGCACGCCATCAACAACGGCGGCTACACCAACACCCGCATCGAGAAGTACGAGGACGGCGGCGTGCTGCACGTGAGCTTCAGCTACCGCTACGAGGCCGGCCGCGTGATCGGCGACTTCAAGGTGGTGGGCACCGGCTTCCCCGAGGACAGCGTGATCTTCACCGACAAGATCATCCGCAGCAACGCCACCGTGGAGCACCTGCACCCCATGGGCGATAACGTGCTGGTGGGCAGCTTCGCCCGCACCTTCAGCCTGCGCGACGGCGGCTACTACAGCTTCGTGGTGGACAGCCACATGCACTTCAAGAGCGCCATCCACCCCAGCATCCTGCAGAACGGGGGCCCCATGTTCGCCTTCCGCCGCGTGGAGGAGCTGCACAGCAACACCGAGCTGGGCATCGTGGAGTACCAGCACGCCTTCAAGACCCCCATCGCCTTCGCCAGATCCCGCGCTCAGTCGTCCAATTCTGCCGTGGACGGCACCGCCGGACCCGGCTCCACCGGATCTCGCGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCAGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGAATTGACTCAAATGATGTCAATTAGTCTATCAGAAGCTATCTGGTCTCCCTTCCGGGGGACAAGACATCCCTGTTTAATATTTAAACAGCAGTGTTCCCAAACTGGGTTCTTATATCCCTTGCTCTGGTCAACCAGGTTGCAGGGTTTCCTGTCCTCACAGGAACGAAGTCCCTAAAGAAACAGTGGCAGCCAGGTTTAGCCCCGGAATTGACTGGATTCCTTTTTTAGGGCCCATTGGTATGGCTTTTTCCCCGTATCCCCCCAGGTGTCTGCAGGCTCAAAGAGCAGCGAGAAGCGTTCAGAGGAAAGCGATCCCGTGCCACCTTCCCCGTGCCCGGGCTGTCCCCGCACGCTGCCGGCTCGGGGATGCGGGGGGAGCGCCGGACCGGAGCGGAGCCCCGGGCGGCTCGCTGCTGCCCCCTAGCGGGGGAGGGACGTAATTACATCCCTGGGGGCTTTGGGGGGGGGCTGTCCCTGATATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAGTGACACTTACCGCATTGACAAGCACGCCTCACGGGAGCTCCAAGCGGCGACTGAGATGTCCTAAATGCACAGCGACGGATTCGCGCTATTTAGAAAGAGAGAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATCTTTCTAGGGTTAATCTAGCTGCATCAGGATCATATCGTCGGGTCTTTTTTCCGGCTCAGTCATCGCCCAAGCTGGCGCTATCTGGGCATCGGGGAGGAAGAAGCCCGTGCCTTTTCCCGCGAGGTTGAAGCGGCATGGAAAGAGTTTGCCGAGGATGACTGCTGCTGCATTGACGTTGAGCGAAAACGCACGTTTACCATGATGATTCGGGAAGGTGTGGCCATGCACGCCTTTAACGGTGAACTGTTCGTTCAGGCCACCTGGGATACCAGTTCGTCGCGGCTTTTCCGGACACAGTTCCGGATGGTCAGCCCGAAGCGCATCAGCAACCCGAACAATACCGGCGACAGCCGGAACTGCCGTGCCGGTGTGCAGATTAATGACAGCGGTGCGGCGCTGGGATATTACGTCAGCGAGGACGGGTATCCTGGCTGGATGCCGCAGAAATGGACATGGATACCCCGTGAGTTACCCGGCGGGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCAT  _E   7   >      3   5   <   (   )   7      '   %   8   -   1   '   ,   0      @   F   J   '   &   '          4      +   %   $   :   -   ,   4      $          (   !   9   *      )      +   2   (   %   9   6   7   +   .   H       %   8   ;   6   0   4   +   /      #   /   >   7      5      0      6   :   /   2   5   =   6   7   N   [   M   *   8   7   5   &      6   *   "   *   7   6   "      -   *      )   &   8   )   !   @   ,   4   %         (   2   0   1       )      (   "   *   +   %   *   6   8   3   0   )   F   %   /   3   #   4   +      <   *   5                  >   *      !      +   .      (      $   ,   2   %          3   2   )   3   <   P   ?   %   ?   3   4            *   ;   <   6   -   0      /   "       3   <   8   ,      (   7   .            (      !       +   #   ;   G   &   :   7   F   5   =   ,   3   9      3   9   )   *       :   A   -   .   D            +         =   9      2       4   4   '      E   :   .   1   2   ?   !   5   !   :   7   %   K        7 ^ Àš›�‘¥Úë./08Õ
”
Ê
ìˆdefk’ÈÉ”•stFõö*!2!3!4!i!j!‚!ƒ!‹!Œ!�!Ž!�!�!"#%{%ý%±(í+> §ÁàhœZtÛìù19lÖøVcàá	³@ˆ¡QÉ¡¶mÛí—ù)±û‰gœÊ÷+!5!k!o!W"##˜#Î#V$N%|%þ%û&Ö(¦)E*°+}, $ _ ü €¶ÁË
Ñ%ä?l|“Äþý9G' M Î"ï"l$%²(W,3 >RôüBmz’¦å¼BÇ•
ÖÀR? ±¤°¿
Á'–ÌD¬#u£ !P!„!‘!#¥$‘%C'ª)*î+…,5 ¨ï8ùW†	˜á<
¬
ˆ0…°¢ßc²ÂüŠhËÈPn•«±´ÕehdÜ.3W6!C!H!l!X"$#}%§)< œÂÜáií:T¥ÒX	â	0
i
´3³Ü·Êä·ïóÒÜp�yú*p�K¾½_ ‹ ï '#P#™#($ø$O%	&.&´&ü&1(Â(*F*ß+f,~,Ž,( 7 �uú2Â×
ó
©qn±@øfk!9Û• ,!ƒ"â"`#Ï#õ#W$y$Ž$ÿ%á&º'‘(×(ò(±+þ+) .[Ümídûv	WsGgAG‰¢Rî˜¢ívÓ`*ì@Z¦ø>!p!é#h$ˆ$–&(7 ‡uñ Sú…ÔÌ
Ÿáž°ú½JPcê2}ŠÅy¡»¾$]]Œ4OyHœ³ÇÈ �#µ#6$K$%…&+'Ô'ê'd(‹*g+X, ·F”–÷¬ m$Ï%ä%'t'Ž'³'É'(=(^(ž*' % ` ý Â¿¨É´› 	e	
4:©iåm³ˆÿY¡çS˜( F N € Ï"B#…%ì%g(³*è*'+% �±²ZÙ	Ò&“ÙA6@M¥œm§þ:Ué= !"ð"ù#º$º%1'³(Þ(:)*õ*8 ?SÊÓõýCn“ª·½CJ¤�½8�¥¨áSz}°IGRvÊø6Q!d"#$P$¢$¦$¯$‚%’%È%á%G&ˆ&§&'Ñ'Ã*�+¥+ï+†,- Õ “Ðæô{®àT	¢ Á<€¬·;@q„± Â(Í!E/x #K#¦'L(Y)ƒ*-+j,1 Y§&Q	~
ŠžÑ×��›ñEŸãj±!Ý/VÈL0$¨}‚± Ò !%!T!…!’!ü"à#u$Â%U&D'ø'«)à)£*' Ë Y{á@IÊÈ–
KSI²`À ¤—r­‰”¬ÂÕî9¤ ¢#Æ#p%&)&;&í'·)*+, …½XX £
a™#1†±}÷ÀèiÂG®tžÑ²BIf4ší m!%#@#^#f$€%ƒ&Ç'W)¨)0 ö Ö‡(L	™	Q
Z
A}âúTVÓûàd~Üý\…Ì«QZo¬5kieƒXá k"—"™"~%ä(Ð)€+, ú»’	=
«Ñ/l-]u–Ö~˜/ê!U#™$%ƒ'Ý'8(0)N*Æ*j+–+@ 3 ©·ðè9œËh±sº×â­
c‰<äü£`‡1³Ã‹DÉS\œ×þ‹uµÇùg�Ý=yÚ 7!D!I!Y"›"y#ó$%Ë%&f&Ã'*[,F  ÝâjAî§Ç9¦±·N	f
j
�Þ}µ¸Á‚néðîý0qT•pzâ7<aÿ(Ö^‡ÔmLn¿£ßd ã (#+#F#š#$ù$µ&ý&å'(!(š()**G*+g,J N t Ï BÃØåë–n;UY	^	¼	ã	1
L
S
ÉÝŒ¸å@€¸Q©ÇÓÝSŽõýh±Ã\my¼ëü$qIžÎëp ð ª!Ò!“"Q#}#ƒ$Ï$P%
&‹&2(‰(Ç(à+ò+�,' C í Ð¹-3c
·
Ÿ[Í4´&ËÁÕ?§wÑû+5k¾Œ ©"k%÷%‚+,—,& (e�ÍV	uÓë
¦ôY¯Ì^œ«xÎëƒŒ7�` Ì!¸#$)$&/&P'Ã(È+' Vž5>Dv£*?b	†	ì	»
Cœj´&©ùg•¸âå¤Ñ»Ì-!m"a#�#ö#z$V+,  8 ø r�Õ3Ã³C	âAõ/ãlÙœ!"Ð#X$�$ & &â&˜)¡)È)²+ ±­cØªÜ›	 
â
‚ªÁ²¶N`	Üï– „"“#>$»'~(Ø(æ(ç)4 qû~B2	\
ô
ø
,B»Ä,Qrqo‚·¢˜f–u")(q¬Üëþ:ç…ö ã"G(’(�(¥(ó(ÿ(h)½*Ï*ý*ÿ+,4, ½ÑÞ€ ï9‰s	Ö	ÔEWwF`Ÿ¢AD”q!€"s#i$c&v&¸(+ K\Ý'3p�¤
¾
tÞBŠSÓX½ÐÔ£Ù»îÔæa)ùþ8"G"ê#!$E$‰$’&“'(?)‰,% /nÎÃîÌô	ÆhH£ðG€ï™€ÞÔ³ÌóÃ‘ "g"Á"d#¬%—&˜'ˆ)c+$ ò d�©eüw	XH“èKóõ­ð4Èéê+zí[Z§º?!Ñ&b'Ò)’+: ,Ñ	òÀTRÒ8†›o ÇâœŸþnïêKdšë=‹Æw“¢¼¿%[^¯Óc7På1–v  #†&'è'ë'e(À(*‰*- cæ3~¡s1ÕJ	?ŽšY€øl²ûLÁ:_š©¢é2‰ªÚ^é6"¶#—$Í$%&U)a+h+Y,, '§MÅÐ?
m
Í
t&úü±Ìš ¾WŒ?˜d¶ª5z É ž#7$Ë$%,'H'“)Œ*+‡+4 y ˆv¡ÖÔéû†Í(wãNÂ#@d¼Q§3~Ûzw€�%I�´Èü 
!2#1$9$L$%Z%À&J'Õ'¾)Ü*Ó+(, 
G	}IY®àÉ!'.'Î'>(_(•)T*$ ‡ �¸×˜Hy&öx“JY�×+¯•—t!Å!ÿ!²$%å%4&u'�'´'2)P*+R+,  G ¶R‰<¬	A
„
‚ÇŽñ+ÀÓø2­­ ½ æ!n$Œ%Ð%'Ê'
((()Ÿ*‰+ ƒ^²O½aî&ŽÍ/A¬pØ€±l+( þ e³Ôµ-Ã5³#	À‰ŸôÌaZš“G C#v#3%A&:(�)?*q*t*Ù*++˜+,”,! & � ˜ ¤ ¯ ‡ª8fÈfô×"8T½!ì!%"Ð"›$Õ$í%((Ñ(C)_*È*é*+(+9 xÃý)©ÀÝ©Êü n˜ñšàf	m	‰	o
­;j1œ6æn�åÑ…ì ›@™ O � W#‡#Ä$†%J&ª&h(p()f*š*Q,* a óœ	”	

Ï

Ó™´ïd˜ŸÅ|¢è ) è +"½#Á#×#ð#¾%Ì&''5'Y'ß'v)W*´*æ+, ‚#0•'¸n§ã_¨ä ;kõ"—""ì"Û#K%W%»%2'�)—*) 8Ì�òzgéZˆ
0ÓðþÚs‘Ï9ÿ‹Ï> ú!"³"#ú#`$»$&ß(Ž*+F+x+¨+ , J²á[uFvVzÃA¦u‘OVû¹×êË ñ"~'´(;)m)4*+ H�³$Çê|	°	Ú	áè8”ÎB‡Ùÿ7NÕ�f”VŠ¹¥�V g !–!p"é&…'*ö*2 5 òxØ¦ÇDR>�^¦©-T{J[û>MSqwŸ7{0!F!R!g!"b"I#Þ#I$N$£$y%ƒ%Æ%Í%â%Ò'b(æ*�+( @TËÔéo”«š·nÜ*^5s'U±D@�ß ô e"~">#{#$d$É%‰&Å'f)Î)Ä*�+ð+‡,% ‹ ¢ F1–Ô¾KX‚
Á¥bˆ‘~¨Ø­Ãiãùæ "±"°$1%Š%?&H&¨& '¦+O,9 f öþ.7D]êì¸Ù“•¿NFŽ5¾9âBw�ÚHŽ°Ë?Aa«9!‰"#$Q$§$©$6%w%“%´%Â&'i'(„(*j*»+6 ;S\”—Ñ$|¯eýòõU¼1ÛíìñÄ=BAÝ¼ó)�F²G J y Ï Ü ¾";$€$&§'ª'À'x({(†(*+.+Å+7 Ö _¦!<ø	À	Œ
Ú
^6EÂtx)	­ÆÊçSy÷rùö¿
0µh¿`�"$&#&M(t(Ã)ð)*l*Þ*],k,u,+  £Ãáu£=	$
M‹,]åEf�¸&|Äf†Î�¶f}‹B"­"Ü"#L#n#$ü$c%h&„*6+<,. h ’çõÚÓ
eyéþ£¥=Ò<�H…²Ù!C…Ã5lºÞ"µÐ­!L"y"ƒ#¨#L'Z)1+*,H  Z�£P•Ñ¸ÏOŠ
‹Ø�žæœ5<Pºò3¾GäõXµ8ß/žû‚!·É©!!!Y!\!“!Ù"#h#k#S$v$Á$`%±%Ã%D&'@'E'f'([(À),  ø<„_Œ•³R	 
Ÿb!‰0 A0W“™Mâ‹"# %×'ù'J*}*% ¹0ÿÖBœ
j·*ÒF kçö�É,Vƒ$J² †!á#«$C%�%Ú&'L)¬)¤*Y+8 ¸ G¨­ºjë'ï	%#©»-²"¢ÀÞú1%'~	8 Ó &!U!["ý"Ü$8%•%¥%¶%Ö%V&y&¸&Ä&'9'(A(»(Ù)á)“*®*í*›+Õ+; Ì ŠÇ+9žÍè
—‰3ºFæ;JViŽÚyÁ¡¥ª'®½ÀÉJa€•­Í:C‘"!;![#%$.$3$}$õ$<&×&ÿ)*B*+r,6 { QŒ½âŒ¿ˆýjÁä¯
Þ
ý
!LM®± [MHi Gûeyú–ÕÜñï| þ £#Ç#\%q%&*& '­'$(û)[*w*K+½+0 |î AJ‚É¶	6
�
³
ö8Z[¢\1ú€·ls|³Ëì;a>£âô‘ÃOÊ,P"4#¬#
%%í&k'î'„))*4 Z«`m¡áY1Ë¼Û—
þP]TÅP5O7>eäñ1˜s†ŠÖß KcŸÃ† ¥ !K!_!#.#þ#?%[&^'¸)¡++ # ] û =Q¦ßûAl™ÀYs�bà	$‡Ú~ø>újÃ¯¾C«L ¢ n!Í"ü%Õ(©)*|,„,/ Ûî¤ìúW	h
«
rF2„²îò°xÞÁ uÒ³JgÛ¥^ î á"&#_#g$&-&•&¹'(�(ñ(ý+e, †¾ðY³š

3h¯ùOé‰•¦º3›E  Ç A#œ#„&'0'È'# Ê X©?HIÉ	P	}
‰ŽD;¶HàŸÁ5Ñ Å#�%à%&(&:&T&¦&Ð'X)‚*Â*i,/ ¶g°ÿ`¹˜	P
Y
ª"ÐãûUkú,0|Û¿C„R[­ÖtHfÙ ì –"˜"š"T#%‚&Â'Ü'7(M*> M ì '×äUm¦b°¶M	
K
R
e
~|·ÈÔ¦üg”®°Ðáê#4þÕ]†Í
6l�jÞo â ©!Ë!’"¨"*#‚$ö%&(ˆ(™(*+�+Ç+7 ÷ =CbqŒÔ}©)Û²1	a	š	ë	[
á
+BºÃPpi¡
—e•”Ø'pÐêý„ºî"l"=$Ÿ&}(œ(å(þ(—)æ)U+, ñ &ˆ¨Âó	£
ÝÒWÓÝ¬ÓDéYýÀ"’'Ñ)5 x årÄI	>
l
sv÷Æù›Ëý±ÿmMVÁé"?K9>^c<¡Úv0 u 5"%%ç'¿(T)½)*+†+Ò+ GQ]~±;¼«	`�’.ÆX­*ßo×È!	(')1)O*S*
+k+0 w²Üû,“	¬Ò0eÇó˜œäÐ„`š—™7’¼!ë!*"V#Ö#ï#š$&'Þ''(9()B)€)V*^*e*Ç*+—+, ËŽà#”é{	àçïþrt�Uâ^
NÖf ù!#J%~&„'3*w+6 4 Š èñé0™ÓÆ¶WmØ’¾Û=]rET],×vúhpž>zÞ å ó 8!E!f!a"}"H#z#$H$c$Ì%ÿ&Ä'a(e)*å*N,:  ‘:–dtô÷¿	‹
Ù
LdŠý+\¤ëðswÑ DÉŒ»Øõ…¾Ý´_e| Û K"œ"$û$&"&g&©'¿'w(z(ï)0+Ä+;,\,/ · ¸�^i²AN›
¶å;aˆ2W´¡.�Œ’+U¶ÈIáZ"Ø"#À$%_%Õ%·&'?'@(I*­*ì*2 ªÆ_l�Ìàü»Àã�
®
²
Ý
LZ¡­2\[°ÄEùÊÙðÿ=d…ÞÂ{ … J!-#ý#$$ô$	%]'¬'#(ú)þ)5 <¥ÚÞík˜¨ï>GÈ²ùO	V	ß	g
 gƒ�ñÙø=µùˆß¹2ÀÚ¤›#ß%&'&9&(�(Ô(ð(*Á*ü+h,{,ƒ,= ë &ãBp‹¨§Á¸`	 
O
X
d
*¶¹ÂÇoù	+–{¯”é3¾ ×ÏÕésèíün Ø ¨!"•"§")#S#&ž&‘'6(˜(›(T+6 w v±ÛFß":èª	k
ß~ö€øþ‘LUÀqã8¬Ïbsƒ�)_ Ù™nMe »!Ç!ø!%}&æ')&)*R*	++v+Ñ+7  ‰ �Åçkcû¾	Ž
Ø
Ü
‘*[êY ¬ï1rvÿ[ÈVD -º„oÁ^{àä ò J"`"#,#G#$ú$%¶&þ&>'"(v(H*ä*N v ê %;�¤ÄÙæì—oŠEj=!úçøU	_	½	Þ	N
W
c
×
ÿÆ÷X«÷�øþ“´Î2Ur‚CŸŸÞì]zm × ñ ÷!"”"R# %%Þ%&&&&8&�&5(Ž(ï(%)ã*Ð+z,[ O P u › Ø é CDÙš%&'()*/07M<M
T
U
V
67™ÊæçõöA¹ºRªÈ�ö÷²’ÌÍýþJ�3±â«!ö!"/"~#&%&6&7&o&p&3(4(Š(‹(Œ(�(È(í(î())à*á*â*Ï+á+ó+_,`,m,w,x,y,M Q Y ‚ œ § ² Ð Ù ä —#›þ8>Ÿ	2
§
€š"r»ÞG�¹;B��ßyÉÔÞTeis*³Änz½ÿ4J²ÏãYmvÈ¬!Ó!ï!0"u"„$Ð$q&)))n,�,* U EÚ+1NVZ	ä	
8”ËÂè!»S]ì%K{Ù—¡ " q #Q%Œ&O(S(É(4)õ)â+ô+a,8 D 4$¹õ ?.d 3
¸
ð
<›Îó#5ßHÖà½ÄÊßHjtm¦{-5K³)PªÉv"ª"B$…$%h%)I)Å)o,‘,7  R Z � Ç î Ñøû4	
s
¨
o sµ¸'3[zŽ©zf•È¾6Zbw ­!¸!Ô!Ú!Ÿ"$ç%ò)b*ƒ+«+µ+€,˜,5 ƒ � ¨ œËÿ9{º  	Å	�Ö\¼>ºÌÝ<C‚ÂÕUz¨¤´ìo tän¿Ò� v!ß!¶"Ñ$â$-%	);*:+& ³ Ñ Ú å r˜u��
]`�@”x+ÅÒü,‡lÐð!"1"Ó"´$l%ø%r&) )P) )5ÎºÛ
[	°�¬Üyìû�Pø‘a €#²#t%&Ä(n*º*ã+6 V W,J(WÔ!]Â	'39§éMV}Ãé¬8ZÎÍ^&GLÚÌM_ÁV· r ¥!Í!×!$ì$&¶'P(b,* a2O+IŽF	å	
Ì³+õGaåŒí|è_˜„þ ;¢# ¹#*$#%w'ò'Ê(5)›)*É+õ+0," Ã Ffžgvì
O•"P‹¼Tƒ�Ï8jÔA $R%0&�&Q'‰'T(ö)r+,* W6jžºö@$c	.
ñ
ÏœÏEI™×k¾k•ªahNl’¼Q¯ N#÷#†$Œ$¸$¡*%+++7 1 ?VbÅ+7q¡=@¹
¼
a�à$~çµÅàIun§Z|ºú-¹&ãæÆÒx¸ÊÐ!è!b#Ž#‘#C$%i%â().)Æ)Í*6 E Ÿ%¥w!/e˜çsk	‡	®	†
 =DW$™6mìádŒË×G)–’˜§´¥�«·Ín"&Ì'×*,’," 5E”W¤´	í	4
/ô6;'¸ó.6LjY*.!"w"«"¯"¦#{$J)I+W+‹+p, [ È ï ù ÒÄ¤´©
pti–BvÜ<c("¾$R&'~)c*„+Â+- S â sŽùüCa	

¶¹(<{ªÝ’…0æÚ×N[x£!¶!¹!Õ!Ý!"þ$&›&™)ó)8+³+�,*  = ‘ y¸Öå_5¥D	t
¡ð—4ãgÉöø�¿ä$mÕ / �!®!°!²!´!Û! "ç"Y$*),) 9 ó4…�	v
�\�{!V7Ñ#$�$ï$è%¡&ã&¢)É)¬+¶+™,)  ) „ {�ì6Ý:	ƒ	!
ƒÊÞ=DwåV³·Oaœ…uðDÀŽ © D"p#Ò$.%Ž%<*z*Ü+&  "‡®dÈØâ"|T‹˜K§	ü‘?»˜ÖÚÝÓw!à!?$[$*%e%¼'(ç(
)8*8  ? I l “ © ¼ ²ÄÌç:Ù|”*	Æ	Ë	O]á«½Â.B„Ø&ˆ{©<hƒí
påo "Ž"¢"·"(Ù(q)è)) ž MT÷ «»j§!	?	œ	&
C
Á
ã
‚Ë×eÍƒÃ+¥µuú�— …"”#ã$©*;+{+! Ò ™r®ºü’õ
-£-*Lrh­Ðñö,†ã!é"##m%s&{'�'†*þ*@   ´ æ Njsv‘¶>wC‚	3	F	
^†CL­¼£Ë™’Óý oÒ~JìÑ4h§´ÛÀ!	"2"Þ"ä"	$µ$Ò%ù%j&ê()Q)ì)¾*+Ù+, ƒòù
ìRa_‘Aƒ¸y©-g—Áv#(Ý;¯åŸ!ñ!Å"Ô"#!'(“(¦(ô(!)`)i)³) ,5,>,4 Û Å�ã^6	]
òoÅsr‡p•Éàú,ÆˆÏTÂP)ï|ˆÿmè 1 ¿ ÷ |!p$Ø$ð%p'H(ž( )z)%*/*Ð*N+,% ¾ßé÷6ÓQt	×	UO‘?±züŽQxG’ŠEU"�"³#j$ê%ö'¯(¤)®+Œ, ÏÜ\	¿ $óX3rœ Bwb ñ$d&N'–'Å(Ÿ)»* *6aÏÒû�…‡ðÕ­í‡a£•r!Ã!ý!t#…#^$Ï(o*ä+( Š»NZ¡:Š“žÝ3Tå?FEùà³Ø_!"N"¼"�#ª#u%&w&'‚(¹(×)‘*2 ?.]Þ^’°I
¨´N^Ä­û9[®ÏeÑÎÙMS%ÕSb¼¸ \ s {"í#"$F$ô%“&·'Ú'(@)€*c,0 W 	-K)¿Ã	þ	a
¥
Õ
(4: e‹TY~Y§¾¤EH¼	ÍNç‚àWktú¦!Ø!H"$à$ê$m&Q(1 ; j ñ`�´oz’"qŽ�	,
¿
ê�ßC	cÖê	�)ÕÚX_'	JhWô*ÿz!Î!Š$í$(%&Ë*À+  %LXë(XÕ4ü	u¢¤WÔ#13CÛïñZ`Âß9"ë#è$”'ü'Š,) bÄï‡ Íg¦°´,ÏÔ¹Ö�îé`™®…ôˆ$ ’ h"e#º#+$<%©%£'°'3+C+d+1, ^”õN‹�:ÍÝb�æ9�PÍÂ"˜&x'ó'þ'œ)( íPowJ		Ï	õ	x
wÇ¤ñ¥ª®ðHö}%àŒšßð£´n<;"™'6) *Ê+E,"   03Ï,Gæ	
iI+H5ð�}Õÿ£Ä"$%­%Ú%Ë(‰)!+n+ö+$,›,* ó GwOýQi„„ôÐ®5�–ÅãþÄ$6{áîÒòˆŽ¨@!?"$1&Þ&c'X(Ë)Ý)“+¸+,+ Ä g"›Â	x	í
±I–”#Œ(UžS‰ŒöKÉÛ\Ú\B ‹#Ó#£&U(F)°)÷)s+K,% ~ *ežÜõª6fŽ	öë¾½LÊÝCÏê÷#7[k¯Õ!Ê#S%å&+(ú(\)* mŸ'hçZ×¢	YP{é>ñ‡´'9b€ë,™·Ð»$’$ž$Ž&Ò&R'Š'Ó) ,,,6 ~gµ-k»÷AU	Æß“
Ð²Õ È µl¿ãëQ–(°‡>c›Ç£	À&Ë8& �!Š!#Í#U$%�%B'V,8 -
7Á…Ñ
/
;
ò
f‡F¨Ûp¶ãÑoŒ?¡b÷eoìÇOm”_°ÔdY½2VÚ¼” O#x$‡$�$³&Á(Þ+3 Ÿù„Ód	9¨žà�Ø¼l5‡IL¤›bx ½#\æ²öR—è< « ø#¹$²'é'(<(](f(Ý(9)*Š*�*&+f+0 XÒ’óz­ß%S�ÐœJš¡ÿð«žâi£:Œ°Q“«Éíw ° #¡#t$®$F&‡&'¥'ì'¶)¢*+,+) 2  çWr¢ºÖK	‘	@b|;áù.}çF\ýŠœé!j"x#˜$%%&‚'ã(/)V)i++,Z,F  B d@ÏtÆ,28»	¶
�žZÌ�%?mÀè>P¨Æíü/RXôvoÂË;[»û`']›ªwê‚‹ÓÍêÑ!E#·#Î$j%ä' ()–,% 4¢>A…	º
›�À�³¶áµôM_¨.·âáä£«ÛË›!�#’#F(¤(Ç)Î*3,/ ¼ŸcÍî8œŒËr	Õ	½
Å’ïFçJÏØòºÓï3Çè_y¹òÂ� "7"F"c#r#D$«%b&·(>)‡)b+3  &}¦Ó0ÌQÏÑ>�7X…%'šnûâ™îc‹»À—™™¨µv16è•Ÿ#8$Ê$Ì$&G'I'Û*`+# F † 	ÖµxNõ	@
wí%�ÌIX|H��ð*@«1¬%'-'Í'”)ˆ+,4 — ® d(¨ÿ—ð™ßl	ˆ	
n
Î
	7²"e›¿ÖžëóËž{?  ¼#Ã$Ô$Ë&o(>*s*Ø*+
,“,+ + "fè/tÆ¯	‡
EuU7ýyÍÂØÔe“·Î¦¸œ¸ÎU Ê !•!o"V%è&l)�*+E+ 6\w•×¥�
)4M&‡AZŽ/!"°"M$0%5%‰%³%Å%Á&h'(i*Œ+< ¥ R#Ùüñ¢<	#
»0Úì]xèä5D<(ÃAeÅæRxöÜB„kŒ¹	µFŠI Î x"¬"Û"m#§#:$b%K's(Â)*Ý*5+),* ¢FÕê·Î‰î	Ÿ
i4O¹$½ô¨/7õùú� &#7 [!g#j#C&Ö'(K)¿)|*X+š+Ô+5 z ‰X‡iµ	5
ç
ü
õý7Y O¹0Z4h{ëdxJžÉ+ý !!!^!3#Z#-$2$|$%>%[%Ö&ƒ)A*v*J+ +q, \ É ú úŽH	ª
qCwÝ”¡ Ì"S&'/'Ï' L ð µalÓ¥¯µê	¢
ÏÑhj~ƒÒCÊ!‘" (ý(–)L* P�q+ušÊ—èJ=á‘Õ)")U*d*…+ ÅµMKZ¯d„ e!×"¿$'¾'?(`(ë*Ã+ ˆ °Úb©	õ)ŸØKZØ˜º!Æ!�'Ó(Q*+S+‚,> T � š ¦ ± ã $Ž–£Ø‰™ý$Dž÷	T	
þ“ºÅÁô:Þöd)‘1Ø2B°–ž\y l î!õ! "t"ÿ$%5&œ&)))$)3)ô)Î+* � q3t�Ê÷úz
r
n·=y“”£†,(O
 u!·!Þ!"Ò"A$³$%,%g%æ%O):*a*9+´+ Â ¹fI'�è|²«äçÛUÿ:¤!Ö!±#"%&v'µ'ñ'š),! UVi6—<j	­	…
˜5l˜}‹:ò,%‘¦w® ç!Ë'-) **+Š+ á ·äB^`u
ï h÷�;å#ÔÖM. ¢!¯!±!³!µ!Ü!'"š&))+)+   ( > H ’ !†zÃÇ×áæ!SŠ—¦)	Ê	B
ÊAƒ—äÙ›ùC �"¡"Z$�%(p)7*.  M­¹=E	¢ñ¬ÊÈù‘ÀÎnÁ3®³Úä0 ¾ ž!¿!â!Ä"è"#o$×$Ñ%ï% 'o'z'œ'(é(_)ë)$*+ `‰„~5†PT�#>2q›²v»"]$ð$é%õ'�(®(ž)£)­+( : >-êð_€³n‘¾‘€	+
Ž¡d]bú�"(0dWB$YR�ßVy!Ù'û'*Ê* ôMv9†Î	w
©­ï|ÎÜß‹8ï~¢­OB+m+D,š,$ šô	�	¡	°½h'ƒRˆBÎö&•ÙÏ>"Š#Ò#$‘$�$¢&Ý&ä&*(E)¯)Ê)·+,, }f´y¬øƒÔ§Úß´
‹â>P† �£án¢†bÆª ‰!­$�%(;(Ü(8)œ*+Ý+2  A ÿ »žÎÌí7›q	„	º	Ô	ÄË€¿î>E€§æW´Êáv[›ñÁ� "E"D#q#w#�'ã'E(£(,•,% * … – ­ Õ|þÞ.	6„x$ÁHbŠ�ÓòÍ·
ç0>”›Ó$n(=*r*Ú*+  Ø¶;	"
ž
º3¸ÄõêøE6 H Y#/%4%ˆ%B&r(‚)@*h*u*{*™+Ÿ+ K ´ŒpLJ™ÉgÔƒ   �"½'ê*3 Ž ™ ¥ ° Á #2¯eˆÉÙ#�¨	q
mçý’Äô±ó9ž×õ�þ'9	  í!ô!Ñ"°#@$+%f%Ò()))N)9*`*Í+2   ' à ¬ÂÆàã TUh–(	i	É	É«@–Š9ØšÍ"¥BL²- ¾!á!&"Ö$î%n'›'è(ê)#*+)+) ˆ,éï}™óŒ	 	Í	S¯¨¬¼gù'Û‡î#ÞUx!="º"‰#\$œ$Ü&)(€(D)®)É*A+,C,3 @ • ¬ | Í«Ëýš	p	¹	¹¦¾2¦=…œ¢Òm¡à¶é	æ/šˆ!"X#‡%(m(q(¢(Û(g*›*<  J À ³+«ÁÅßèSoò•œh	‹	È	Ì	p
Rl®Ãó§°ò•ø‰�8†íœ&A  ‚ Ÿ �"¹"ˆ#¯#))é)"*Ì+B,P  	 ” ª « ½ ¾ ¿ yz{Äþÿ*ªÞRª™}›g	n	o	Š	Ç	÷	Å
PQ^k”^_¥7çèŽ„Xº»óô€�‚ƒœ � ž ´ µ "¸"ô"õ"ö"7#ã#K&L&i(j(k(l(©(Ú())[+A,? 
 m Å;qÚ~áxî+	ø	Æ
<_âl¬/C…Ù'`t|ªéo�žiæîqY�¼õp„P ¶ £"È"÷"8#ä#Å$E%M&«&ð&	'ª(r)\+G,R,% Ÿ N�¼@	Ç
Ð
­†jvö™.X ÷Æ}†"•#¾#Ø#¿%ø&('6'.(s)X*ò*ê+,?  UôøL­�ï	•	ù	z
$ƒÌØù,0DÚ(b„Äa€š«p¦µj¶r©‚0í�¶à:på…q…é ,"¤"Â#Z'÷(w)¦*ª*8,H,3 <?¬k¨	"	%	�	Ñ	
D
Â
ä
!y=`ÔfÎ	,}ïZyÆ ðeRsûŽ* É"ñ&
'à'«(<+]+|+ç+S,4  b n 	ÆrÛDây,	
'
	ãm0uê�Ÿç½>Ë¦£éRñ Q ˜ · !ø"9#å#ñ#Æ$ä$F%™%Ÿ%N&¬&Í&µ* Ó šs¯–È
.®o®!-‡ö€(!í" #–#L%n%¸%À%ù&)'/(ó*ë+ ƒI»1A	ö
¤@kw©sšiå÷'l˜t&, ñ$lç.¨YÑ#ä!#"ê"¿#Ù#X%¼%¾&3'|'t)‘)‡*•*˜*ÿ*&,*   O‚ý“€½Ñ
(‡!¹+Mä÷$`/¡òø>”»Ç<~þW!‡"#Ü#£%¯%7'ž'Y*; ! µ OV{?®êG	–	ñ	
{
‰
‡Ù DcÅ¤š¬ ’pÓ:¤×î�;5Üê 3"ß"ÿ"Ã#û#
$a$Ó%ú%€&$'['½(R)c)í)«*+9,<  ç 9t5·óD[	%1ÔMñK1EÛÿ)»�›ÆÌqkª	üK·qæÒ¨ÆÐ? Õ -"¥"´"Ü%{&–(ë()¿*p+©+ú+6 / º õw’�hIƒð	4	_„Íú-U…Ûb†:§¶t“þsƒƒÐí¥µ
""å"#¶$¼$'à(x)§*#+G+y+×+Ú+�,- kÍùÑMxžú	G
	®½¬·Ô1á †Œir†Z Á!û!"S"Þ$&%:%§%Ø%k&'Í(ø(Û)�*+I,0 ³ó

9
å
ú
zûSW B-zÍòð.v®[É$fWØºæ: Ì #((µ(<)j)´)^+}+T, K@„\­l)è	íbÏ{'|’St�Ø !Ê"Õ"¬(")n)5*/  =v	#	&	ž	Ò	Ã
"å
`’~h˜§ÂwñÞPü°+ ò!Æ"ò"—%'á'C(§(õ(?+�+è+,6,?," - â©E
G>awùÕg„¹ÄªBzÇ)+à<üëÆ&ò&'"'”(a)=+ø+  c ª¯´.%D½/´‹ºð§é^òù"æ#ò#Ù$š%X&Î&ê&q'I(&*°*ú*O+3 
2DÜä�¡_7	±	Û	
^
Iópyä9ˆv‘û -8‰Ö•Qâ*}3ž]"å$G%ñ%O&;'†'{)ã),*0*,<  säí€Èë}	(
âcé×it•-q–ˆë7 ÊáÇ%Ož«ÐUÃý-?‰ÆêSR W ™ q"q$Ç$ %È&Ÿ()*ï*÷*8 o Ü IÇ‚†¼ÆEãz«-	
kÆnsÏ1JCÚègW¾oÂÌ n¦¤)ní  2 h ¸ À ø !!}!—!:#­&º&ô&‹)¶*Ñ*, ¿óy¤Ùäêø7ÔÉ
?P{‘ý°“ü"rEô!)!1!O!h!V"î"!#—#k$¤$M%z%ú&°(¥)D*¯+ì+ 6 ›S—u	V/¯jª)8ë?c-Š =!B!G!‚"è#ô#'$÷$à&0(*�,( t°±§ÈERÿØ	’@²1X\‹NTx Æ "´#5$J$„%¹%Î%ã%ë%*'s'�'Ó'c(²*ç*ô*7 Ô Éà¶Ïp¼Ü7_ƒ�§.RUy|£¯ÇHK /q.Fˆ§Ô÷8|�$!S!c"û"J#ß#$O$¡$o%Á%Ç%÷'K(ß)Ž+¤+. õ „Õ¼›Ê'SÒ_†ö[ÁªYt›4js�ÐÆA}ø‚—<x™à ?#]#e$ò$Ê%e&Æ'Ï)
*Å*•+ s Î AÌê•¸]	ê
¥ÝÀ‹S6lxHm¢c |#$$Š&O'Æ(ñ+ U°p¬B	÷
A+%t(æõ g) )¼*ü*, ÐÝJ2o¼ôV²åEž¡Ë(C“f"" $u&‘&Ð&a'—'‘+ˆ,( +bÐ2Õ¿Lè™k¦©è’ˆ©Ù Z®Òbä$û 	!1#0$–$Y%¿&'’)ˆ*', Œ‚—ˆ%ƒ
Ö®¿Ò°¼ s!Ä!å!þ!±$‹%3&Q+! Œ £ Óòü†mÂ5îc!Äç $"u#†#À#2%½%@&I&©&4'X'Ð(u)p*™* +å+P,  7IG€ñyY/†8‰�¤ãjôú!Š–"²"ë"Ú#_$}'�)–*§++ e ¡ -EëQ”À�4a§C¬?œÂÙ=L�¯â@`ª"ˆ"=#Ý#¨$v%x%>&ƒ(Í)º+# g ^[¢Â;Ò
Gø%{Ãeò4´�±Ïg¬¾!A"½"‚#$$&…(*k*+t,; ÷ÿ/;ƒO‹¬¹þ””Ð) "FŸºÞ,¿æ@˜ !X!Š"#R$ª$Û$7%B%”%œ%¤%°%µ%x&Ã&Ù&''8'e'(Z(º(Ø)’*,G *8P‹¼í 0�‹¾Ú–ˆON¶å6:=LUkr�:GhãFú0x¢©áóù&�•¼¿ÈÔÛðI`ÌÿBNb�:!O"#«#Z&ì&j'Ÿ'(*Z*¼+& " P@W¿rë	
|
ˆ2E®í¯:N¥½ª
D K ] ~ Æ Ð à"Ä#û%&,&”&¥&¸'�*d,: <T%f|þa¯Ú_±0	—	ò	J
à
©!{ÜO ¥f“­Ò/ÏÚ"“ÔB	&ŽüG¹Ýë ¿"<$�$õ%�&Á'Û'|(‡(å)*+Æ+7 Êä\}°“ÃH	z	ræî_ÅdÆü°ò›Åý!>W];TÞ	6 t 4" #Õ#î#I%%'(&(¾(A)S)¼)2*]*+,F ¶ Ž/]^•˜Òß@óöVl¿Š
š
±
Kµ½Ú:\ÐCqøØï+‘Öô*<Tc„½Ü³H z Ý |"ü#$#$G$b$^%Ô%!&\'¨'«'y(d)î)ù)ý)¬*/+:,M,5 u§FÞÀÿ	�
Û
)fŒZu*¿®«Çè
Ž½ø,¹Î¸èrçƒ1ÙÀû§!I"_"¦"#|&='u(—(À*u+û+= X × è :".6Li
= ùæÝ	b
¦
Ö
5˜!qF  ŽWxr
³ËITz€ÝáXlu Ç! Ö ."Ý%&$&n&N(R(ì(ß*^,l,v,,  Æ 
¸ôÿ€	Ä	ï
;\Õò_2ZÜ¨¼ÃGyÇl¥ësa©ÑÙ!ž"µ"$á$)H)Ä)ñ)ª+3 4`*H E\	Á	&2NLU*OŠ7F‚œÍ`‹F
úýËLO^÷À¶iÓ@ $ë$s%ˆ'*m*¹*q+/,9 0 “¤a�Äpæ#r³	-
ÿ
`Î.VD#ëæc·ÖF”Y`¹(—MkÅiœX‘·¶Ï!"®"M#�#¥#‹$·$&á(Ì*Ö*$+H+ < xò£„�¤	
Œ–;Ü‘uÛU„æ"½$î$ý$Q&'})7+Á+3  k » LSöë5i{“J9	>	‚	¦	%
À
Nû�Éà
-d×%v‡*;g‚t„~Œ¨ "C"o#)%d%y*¨*z+Û+9 iÄ‚‘µŽâ]vñ	5	…ëKn^†
¨ßÿSÑ
K'O}Igî{‡Ïðõ+g¦  {!Ý"##$i&y)²).*…*M+Ø+=,) ÎúMYèöÒ’¾ÿN>ò2SäD†ß‰×^!Â!ü!"M"T"„#©#'M'•'Î(Ö)�*‹,* i $y~¯û	ý	H
`
Ô
³£XÕ¦­2ØDðIRg»Þójs[ z"ì#ß$ç$é$'%ó%l&¿+  ]“ìŠŸfv¥¯¤*$Ó4¸ÕŽm‡:";%¨%Ù%¢'¯'ý' +2+#,: } l!&)æÁÛNYÖ5õ
zê‹ÉÜ=J€†³Ú"[aÄâý6Ã#5˜¶àÑñ‡�® !É#W(ù([)Ü)+,J,A ‘,„ÞÅÐÞ
:
’
e§±Ÿ�µÐ»4'Hö¯9aš¯Ÿ"ÊXªÈìU±Ùõ»ç% ; “ €!##Ì#T$s$w$%E&²&A'¤'±'\(µ)e+U,- ¹‹Ê�	µ
{:‘-¿=Oì.QóÎó^×ñ¶Ò\àE�îŠçüÌš!i"ª%a&(¶(=)†)~+2,. c´–ÒïËPÎô
ŒTv6!˜Õí{º¿ŽïêÊu0 T !”!»#Â$É$U%Ê&ç&'F'k)D+_+	,D [¤Q"ðhˆ¡€
æ
û
hÙëüX\ç4CN/3åYw%g†ôcùAƒ
@‹¸"‰Í  !Z!]!Ú"f#i#l#,$=%a%²%Ä%Õ&g'((Á)*4+ ù`k�O®´*é	¡
ÎÐç‚“ÑB�d!Ë"Ö"ÿ'ü(K* € p•¢öŒS	Àc¢ã(1ATk s"%!%ð'#)  L…A–];îk—|:�vÙã¡!Œ"Ã"#™&y'^)o)6*+ _=Lu…m�8	*
 cÞ"c‚ŠQ}¡AÍ=õ%1X”šNQÎuØ'ô'ú'­(�)~* ºx×÷‚Ó	�
ÓÙíŠáGÉÃàñÅ÷=5 ¬$€'â'D(7)ž+ ß 1îg'	Ÿ	Èæ«I&™Ì!¤ýK±, ó!<"Û&m'š'M)­)@+,=  Q pwí	¸	ö	Ä
k¸¥ò¦¯ñ“÷]s~¤�›¡l ßW€¹òµè%.› ³ ‡!Ç"ó"6#®#â#D%ï&'¡(¨()!*Z+Ë+@,F,9  €>CK¬
	$	Ð	y
 #xø+a/i¨xÅÿ/ì=ŽµÊß-9Wo¥äöQQr„ð!˜%ž%÷&-(ö(¥*ñ*é+7, ðHkæ? #=“º&ý'!V!¢%®%·%½&”*%,2 . ¹ 4ÐHð	F
TJ…ºÅ9«‚~£Öû¤ÅY Ô "R"þ"Ý$%%9%¦%×%Û%z&''#'•(¼(Ì(b)Ú)"+o+Ö+ù+œ,   ~(ç	
8
äøúÌñ&­{È*ß ×9 –%Å&(B(>+œ+÷+4 1…©®»Cã- ìªHbjxÖh,I6$Cª¼ü.³,n~Áá2Å(]m
ì\"W&¹&Ç&ó&:'Š)â)+*¯*î*ù*4 È£°µÎãþi0W¯»Û6‚¢Æ(.7pêb¦Ó,DÅó‰ !#!<!A!N!ú"ç#ó#$&$4$ $ö$ß&r'Œ'J(Þ) *C*±*£+' r Í ô ¯É&é
ŠRÑR…»õGZÀÏäÅ|Ê�–;’¡\# $$�&Ï&`'	*û*”+ H‹x˜j4…¾7Ñç� ÿ¯âó ‰» ú !0#/$•$2&W'Ž)P+E d ),:ŠŸ«Á/ÏPEµ9<KjqŒgzÂd—¦ñ3¨«%›¾ÁÓïKŒ®ÎáM_f�©½!@"<#~$Ú$A%›%=&Y&Ø&ë&d'Y(Ì)'*+¹+,s,: �¾Éãq[`Ùk¾Â/	y	™
°
ß
Jqzå¯Ÿ’ü.9V!\÷*ƒÛû©	C } Å Ô#H%]% &+&¤&%(»)ä)ø)ü)1*\*,L,. Å 3hEÝ	GþåÜ	î
[—pN‰�VwF_êŠ²�+¸ÊÜ]ØŸ  ^"
#r%<'‡'G)¸*t+.,1 hKRœê4�¢Ã\å	8	¥	²	
þ
JmÈ•":$uÞTR–NLjHz›ï§ Œ#¤#P&|)±)-*x*Õ*L+2 | \ #å‘À‰}®_
²ôM)×ŠÈQRãIüf~4ˆ—ÖÝò]ðÿ È#æ$ò%¡'®'V(Õ)¾+",? !+ƒÝîÊgÉÝ�	‘
´
dê9[u¾3N—ëMì]&2ÑDt€íób‰æø@	?ŠÇëT°Ë S !™!Ë#r$È$%T%`&±&É&Ô&æ&…)*!  o¡jKt7~	)
j–¿¡âP@�@ÌÐ<ô$uj c!r"%ï'û(])5  Þ fö�«ì·	÷~Øì.\r£‰ÈŸËÞ VÄþ¸.´Äë<8¤ãõPq-ï4 š !5#­#î&ö&l',( ()ð*! }ï„BŸå7
ãS÷8Ë¬ûm}’¹Ä\lÖX Q"¡%¼&**ø*: q ®(‰¢ÀÈâÎÐQh6Y¿8pfcÎ2-ošÒ‹¥à+Le ¼Äˆ º ù !!!M!/#;#ÿ#$”$Ÿ$@%�&V'_'‹'�)¢+7 [JÈgpZé3F[Øä|­½.	¤	˜
ZlÇ otE^ÝUéÇQP·)‚šî‡¨× ¦ Ä 	#º)Ô)·*Ô*!,-,% Ý n iƒÜR}2Mê??Ãòˆåó;t¸pî 3 i b!~!˜!_&°&»&Ó&õ&K p ¬­abcdefno‡ˆ½¾¿Çè2ÌÍ{¬£	Qÿ|KLDÛÜÆXµ¶ò(:Í�™ìí§Ñ*do‡ ¹ Á Â Ã !!L!`!a!“$\&]&^&®&¯&S'T'U'Œ)¹)Ò*Ó*  9*  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        4                                                                                                                                                                                                                                                                                                               A                                                    	   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           	                                                      
                                                                             8                           2                                                               $                         =                                                      
                        '   /                                                                                         	   -   (                        	   	   
   S   0                     
   	         (               	                                              &   !               +   E   2      /   9      4   '   4   9   ¹   –      <   5   !   ,   3   �                     4                                                                                                                                             A                        	 
                                                                                                                                                                                                                                                     	                    
                           8         2                     $         =                  
        ' /                               	 - (        	 	 
 S 0       
 	   (     	                & !     + E 2  / 9  4 ' 4 9 ¹ –  < 5 ! , 3 �   ©™  «s  «—  «÷  ³'  »/  Å3/s	Ðg"v&È]®ô :‡ˆ‰ŠÇâã«äÈ|Š–Ðû67�¿À@p´¶·—¨«‹Ó	*îï 4 š !!#!`!a!b!c!r% &2&Ø&ï'�ˆ)Vá(	
à
«'Ÿ¦7à+ð&‘+Ë%b&QAJÍÿ),èœ*U"ánÜ}%í­«�@š Ëé.0QoGSºØA¦¾ŒÑOÛ—
ý
Òí<FÝ5MsÞU|©Ù¨,5DM†ÀJw©ÍÖ:­¶Ó !0!#£#þ#%$§$
%6%Š%Í%?&{(ÿ)\§ ¸| R�B€•"ùX$ˆ)Ò)‡�â€•"DùX$ˆ)�€•"ùX$ˆ)"""""�â-

Üg¨ …*$+‹,Ç‘
Ç+&ç(Ã(,¦@ÛàT"nÜ($Ž$à÷ç'6ô
ë©6 B»ç>ß"C(ëá!Dô
õ
û9¢•%·Ôþ±ë=!¯B Éc”
•–¢ ƒT,¢{ú*¸Án•š(fuÜó(4,pÜ¨~!cgScScà L?HÁï¢”0}éæ#ò#\i*fL÷1k+‡…;2}y8„±á Â!GÊHœÇ%Y)«Ú$3 ð<\ù�yD!Ë%ìn*ã+àJ£“«í8¡#o%+œ § þ8¹	€[»Ë�Á§³nÿãmì.(¬y
aìŽ97,†ï‰8Eá¢GÊø”¥+qêÕ'&ýNæ`hécwü£#§‹×%Y"J'Ó+(,wJ'(,¢ÈâÐY¿Î!'!M!•
R–£ È?$*%e%8*jv³å•
wNR3°Á–I�£ 
!J'(,Jí¡#qn©¦hº•
RÃ –#Ý£ I!Y"õ
æ½&%,8áGÊ¦ÿ¦O­"ÇÀ½ n$7 2Ï#Ž$á&AÄ°™$ ÈZUº)=Q"ðÙëc{:Q‹Í 'Ø'~*õý·Ø½8ávGÊ8!#P$’%'P
e•` ‡œ¨§Ê…cá”
•
¬
WqnARÂ;2}±yÈ8„±á Â•–—!*0G¡ÊÜçHœÇ£ H!%.&U&Y)·)à)³*„:
UHxâ)€'z&•(ù+p+(ü*@é«XÐ)`††Â!ü!¾pq¢£â+EœöP�ÜÝ‘
ØM‰õ&ÀÄLjÏÿˆ¨©KØ	*Dó‡ Ä !L!M!~!!\#Ë#%°&4©"=	$
‹¯ý/6é@!æ#ò#Þ&q'°*ÂÃ)9	y*ÇÍýVÚ5�¡ÅÒ"!$3$Ý)ê“?§$ À²f6ª£8½˜
)‚žÉS$@'ûÂ@Û9$çAƒ
¸p+(£8 "¹%a'ü*‘+‚°ú‡œLj>©¦åhéº•
cwü£N#R§Ã‹3 °×Á–#Ý%I�£ 
!I!Y"J'Ó+(,�@/ _à(qêÕ'&Eœö�Ø‰ÄLjØ ~–%·zÔÇò xŒ�êšÍÔ ³ÖjŒ
œ
¯
Ú
“Z^t1Æ¥8"7ÚöaeC~¡1>ÞŠ©ß?K¿Ï !"!U!L"­"#¨#£$§$?%\%É%&D&t(û)œPMLjÿˆK8£qêÕE'hW­"&†[
ÃPqpn
e•!'ý9‘(œ(ò(þ(þ+,T‘
Kð#= +Ÿf‡;%2+¢â!M!PKœÃòœ ))Ì+¨.´‹§X&ê&&*hY"C	lœ!„:
ì.U(¬y
HxaìŽ9â)7,•ó ¨'y(ˆNùZ!f#i#(¹ËÁ«"–0Z0Ã8> ,cMsñ0Zœ0­Ãw/}Õ?A…×ìš©ë•ªé^8> ‡#¶#J'2)(,/ Í­_êÈÒ	è	
9
_>Dy½Ï
Ï‘-Ð¾©iqæ>éWØº¥"ß"–#æ#ò#L%ù&'á'C(–(à(ã)Üs2ˆ‰Î&†'•	ŸÇÍÚ¡"!ŽÏP:E+KG$^%d'Y(,€•:[#*Q"ðÙë‹Í Ž:^%0z&€'•(ù+ �  Ÿ"ô
qö
@åò óz’+YÖ+,¯¸%ó*V,]~±¼`í�Ì.@«k+Â K¯�¨R&+­è	Ïúó
qn!9â"‘(ò(þ+\
Ärqo(þçö G(�(ÿ(Ï*,¾q+ƒÝÝ‘
d9&©Çë	T°C } Å !Ë#r$%+&¤&±&, áFvÃAûê‡V~Ü«XÐ)Z£P\”—ÑòõU
¼ØæBäX‚ó)·²!Gy Ü !Y!\!Ù"h#k#`%±%Ã%f'§'ª'x(À).+Yz²ÊÇdûv	Ù	•
–
WG“A6RSMœ±¿–—*r¬ìZ‰Õ¦£ ¤ >!·)**õ*¢£âãh¯-o¥+DÄóˆ !!M!N!Ÿ$‹'C*¢+ Ôú]µ#g+X,kM	á †Œø(Û)I,²dûv	Ù	WG“A6Mœ*ìZ¦>!*õ*\”—ÑòõU¼Bó)²Gy Ü §'ª'x(.+Z£P
ØæäX‚·!!Y!\!Ù"h#k#`%±%Ã%f'À)h�\JmÞRNHzï±)-*L+V5b	j©g»ö#ßŽQxGEö'Š—3æŽ®.$+9Z£P\”—žÑòõU
¼ØæBäX;Ji^y¦Á-T{‚¥ó)Jª·½ÀJ­²Í7{!Gy Ü !R!Y!\!b"Ù"I#h#k#Þ#N$}$`%±%Ã%Æ%<&×&f'§'ª'x(À)+.+�+r,¾q¢£âã+ƒÝÝ‘
d9&h¯-o©¥Çë	+DT°ÄóC } ˆ Å !!M!N!!Ë#r$Ÿ$%+&¤&±&‹'C*¢+Ãáu]&|f†ÎfÜ"#$h&„*<,D£ì	&-!z$V+Ä¦°´Ï`™®ô$ ’ £'°'d+Éã[Ây	qå¯üV\Ô#H%%(»)1*\*,�uøf,!`#õ#y$ÄŽâ]5	n†ßSOî{‡  {!y).*M+ð	«£Öþ"#'¼(b)¹�	{:-Eüi"~+ZÛlc@ˆ¡Qí—÷o!H�ÎBÙfV¥g !–!GbQw’Í×	^‘Äiû?„Ô±¹�îéSÐþŠáòˆs ³#º#í#ê%1&(@)C+¹�	ð	{:-I«E£Öüi"þ"#'¼(b)~+äÃH	rÅü°!>]; t 4"¾(S)¼)+Ÿf¥¯Ó¸Õ‡;%¨%¢'¯'2+ 9‰Ev&¸(FXˆiù"±"¦+(‰ÀÎ8pfc2šÒ‹àLe¼!;#@%òxØ>�ûq0!g!£$y%/Îª›	â
hH?wïÑû+€]ÔkÃ– "„"“#k%¬%÷%ƒ'ˆ)ÐÝVEž¡ãCjô!“–"ë"Ú#u&�)–*š{%®––	ñ	z .®-¤¬Íòð’[ÓÜê €&Î&/(µ(<)ú*+}+«÷.Äþ.ë<8¤ãõPqï!ö&,(ð*ÝjîÇ±N	f
‚ð<‡¿£š#(*g,¸Ì~H&f"2^ÿ‹Tú!6"›$u'´'((C)Ž*È*a+,âA§¦·µ¸Án•zÿÖÔ(#š( =#	"`~h§wÞP—%õ(è+6,]ƒ'/ÎhHï€ÔÃ"¬%ˆ)Ì~2^ÿ‹ú!6"Ž*a+ª›	â
?wÑû+k– „"“#k%÷%+9ž;Ji^y¦Á-T{¥Jª½ÀJ­Í7{R!b"I#Þ#N$}$Æ%<&×&+�+r,äŸÃH	fr¥¯Åü°Ó!>¸Õ];‡ t 4";%¨%¢'¯'¾(S)¼)+2+?Å+7�$~çÅunZºú&ÒÐ!i%)ôï	ƒÌù,„a¦µr‚w)¦*t	U�"Œ,½O+e ¡ -EëQ”À�4a§C¬?œÂÙ=L�¯â@`ª"ˆ"=#Ý#¨$v%x%>&ƒ(Í)º+d ),:ŠŸ«Á/ÏPEµ9<KjqŒgzÂd—¦ñ3¨«%›¾ÁÓïKŒ®ÎáM_f�©½!@"<#~$Ú$A%›%=&Y&Ø&ë&d'Y(Ì)'*+¹+,s,5 òxØ¦ÇDR>�^¦©-T{J[û>MSqwŸ7{0!F!R!g!"b"I#Þ#I$N$£$y%ƒ%Æ%Í%â%Ò'b(æ*�+ƒ^²O½aî&ŽÍ/A¬pØ€±l+· ¸�^i²AN›
¶å;aˆ2W´¡.�Œ’+U¶ÈIáZ"Ø"#À$%_%Õ%·&'?'@(I*­*ì*f öþ.7D]êì¸Ù“•¿NFŽ5¾9âBw�ÚHŽ°Ë?Aa«9!‰"#$Q$§$©$6%w%“%´%Â&'i'(„(*j*»+ù`k�O®´*é	¡
ÎÐç‚“ÑB�d!Ë"Ö"ÿ'ü(K*qû~B2	\
ô
ø
,B»Ä,Qrqo‚·¢˜f–u")(q¬Üëþ:ç…ö ã"G(’(�(¥(ó(ÿ(h)½*Ï*ý*ÿ+,4,Ë Y{á@IÊÈ–
KSI²`À ¤—r­‰”¬ÂÕî9¤ ¢#Æ#p%&)&;&í'·)*+È£°µÎãþi0W¯»Û6‚¢Æ(.7pêb¦Ó,DÅó‰ !#!<!A!N!ú"ç#ó#$&$4$ $ö$ß&r'Œ'J(Þ) *C*±*£+0 “¤a�Äpæ#r³	-
ÿ
`Î.VD#ëæc·ÖF”Y`¹(—MkÅiœX‘·¶Ï!"®"M#�#¥#‹$·$&á(Ì*Ö*$+H+· Ë Yª¸ÆY{�á^_l�§Ìà@Ii²ÊAÈü&N»ÀãQ	~
�
–
›
®
²
Ý
Š¶žÑ×��åK›L;Z¡­2añE\ˆ2S[Ÿ°ãWj´ÄEù±!I¡²ÊÙÝ.`�Àðÿ/VŒ’ ¤È+=LUd—¶È0r…­$‰”¨¬ÂÕÞî9}‚ÂIá{ … ¤ ± Ò !%!J!T!…!’!Z"Ø"ü"#-#¢#Æ#à#ý#$$u$À$ô$	%%_%p%Â%Õ%&)&;&U&·&'?'D']'¬'í'ø'#(@(«)·)à)ú)þ)*I*£*­*ì*+0 < á x“¤·äòBUVai�^`£Ä6p—æ#<r„�¤	j	­	³	
-
u
…
ÿ
`Îï.VŒD–˜#5;l˜ë}Üæc‹‘ :h·Ö÷Fu�”ÛUY`„¹ò(,;å#%‘—¦ÔÖMkÅiœXw‘·M¶. ® ¢!¯!±!³!µ!Ï!Ü!ç!"'"®"æ"M#�#¥#‹$·$½$î$ý$&Q&š&'Ë'á())+)-)}) *Ì*Ö*$+*+7+H+Š+Á+ž,*6aÏÒû�…‡ðÕ­í‡a£•r!Ã!ý!t#…#^$Ï(o*ä+¥ R#Ùüñ¢<	#
»0Úì]xèä5D<(ÃAeÅæRxöÜB„kŒ¹	µFŠI Î x"¬"Û"m#§#:$b%K's(Â)*Ý*5+),ƒ � ¨ œËÿ9{º  	Å	�Ö\¼>ºÌÝ<C‚ÂÕUz¨¤´ìo tän¿Ò� v!ß!¶"Ñ$â$-%	);*:+}ï„BŸå7
ãS÷8Ë¬ûm}’¹Ä\lÖX Q"¡%¼&**ø*…½XX £
a™#1†±}÷ÀèiÂG®tžÑ²BIf4ší m!%#@#^#f$€%ƒ&Ç'W)¨)Ž ™ ¥ ° Á #2¯eˆÉÙ#�¨	q
mçý’Äô±ó9ž×õ�þ'9	  í!ô!Ñ"°#@$+%f%Ò()))N)9*`*Í+~ · *6a†¸Ï�Òû^�…¾ð*e‡ðYiž²ÜõAª³6NfšŽ	

›
¶ö3håë¾¯½;ùLaˆ2OW´ÊÕÝ­é‰íC•¡¦Ï.�ê÷#‡Œ’º+7U¶Èa£3[k•›¯ÕIáE  Ç !r!Ã!ý!Z"Ø"#A#t#…#œ#Ê#^$À$%S%_%Õ%„&·&å&''0'?'È'+(@(Ï(ú(\)I*o*­*ì*ä+ƒI»1A	ö
¤@kw©sšiå÷'l˜t&,  2=     C  ,¡        ,¡{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØPÿÎ#£~¾ÈïŠŸ �M`‰QäÕ/…ˆY…&Ó¯×­ówöôX¶¹ØM·j¾‚=åçjb©XAGëE
8IÔxˆà]ý*ý‹AãM5OZ›A¨v×`îJ,wç˜aµí¤â2×DãÓ‹«ýÖÙã‡gK+xÙ®ÿtdQ?OH¾"Lþ=Rú¶âT
Û^º‰gÙZë¦¯Ñ¶ö�¬fÒC•qQ‚©Š¨p}/�¡Š§á+Dk§ý¥&/cê5Ö©©ë¡Âõ@W¹í«wQe´+ŠöW¸6¾vcî‰®ÝDUQÝQAH©fzÐã&-5> ý:9YôøXaÚê°¬ÝSSÑ®<_X�b#kùµf-që­u-uWX„bïMªœÖØMã©dë„V¶ÐâÌòY±tJŸtê4Ì¡é­€µ­1Kî—nf÷ëiPGíöN[ ´@³­�
 «±´míˆZ®øý½"ïE!¢ÙÚWÕ`çw‚›xmÅö…ƒ 8ÑÚ½/�-cîãýþ¢´ˆ8#Üub#>Ø×Fâãã[�B«õd�ÿ¡AÔ8}˜ƒ€z�«YZ†¥E^D®úa�ú¥!q…�¡XÝ(/�ae6­f/e¦­X=ÀuÝgIÚï˜�¾%—RjÏœ/˜8 Þ§§©x§åt€ZäwjÖ‚Wbz•R"ÕÒk¡b™ÿOÕö­xJŽ)ZhzO_MºÒŸ!Fý¢æ­½}[ªi €MÖ] _…­)"üE‚ÙX�…4©Ò‹]³¹kDe¸¢}ï¯ã]8[�{ÿ…_Tú«b‘€o«Õž¡»jbv[â*Sê…SÒnÖXU‰àV”ašáM)P…"•JeˆE™Þ�®ò©�ÂÿX~8V…Ó•™55«­|¾�T–J×Ñ´Z·A¡£‚(»YµÓx¦žzªøƒ…TEMZTnHZJÎq�D�ÞûjC¢R?‰O×�‚­X[‰|Nz¯EJ¾ZÛ´\+cÇR4›î‹ÿo—’ {†fô½§�Vx½Ú´ŽåoDà½#M‰! ?I>#jÖ¶ADª
"fØ¨ u†EZÞ#ˆVúE·‡dJ¿Z¸|@[m…F©-¿X¾ìï€[�ŒR¼à‚ùövØºÅjÚÅaÙo^%Qºh:‚ëROT6›£SUJ‹Ýè8ÔW‰ŽZÝ-BŽ º=Ø˜eEžd‰Ýu]Õ8ô… `êK�^ÕJŸ}KF"êŽû€NuO÷é¾•ß¨f^*z(ÔQï�xýúf–9V87ùSÅc?oªáx"ü´Æë¹êÚ Mæ/b9éˆ\•b,ï&úî(¶ÑF\á†�ÝÇÓï®¶A¼ÃÍA¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  Å     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #´<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="7" name="MET" directionality="1" translationMW="155541.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-5906" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7285" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7888" maxFusedRunOn="7888" detectionMode="exactProteinMatch"><Segment range="7235-7288" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7996-8117" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5952-6163" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9806-10394" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7289-7888" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7888" maxFusedRunOn="7888"><Segment range="6503-7165" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="11356" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="10565-11356" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="11357-11425" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6176-6444" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="9452-9458" color="#ffffff" type="standard"/><Segment range="9459-9476" color="#ffffff" type="standard"/><Segment name="-35" range="9477-9482" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9497-9518" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9404-9420" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9428-9444" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6482" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:51:41">2021.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  2£     C  ,¡        ,¡{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØPÿÎ#£~¾ÈïŠŸ �M`‰QäÕ/…ˆY…&Ó¯×­ówöôX¶¹ØM·j¾‚=åçjb©XAGëE
8IÔxˆà]ý*ý‹AãM5OZ›A¨v×`îJ,wç˜aµí¤â2×DãÓ‹«ýÖÙã‡gK+xÙ®ÿtdQ?OH¾"Lþ=Rú¶âT
Û^º‰gÙZë¦¯Ñ¶ö�¬fÒC•qQ‚©Š¨p}/�¡Š§á+Dk§ý¥&/cê5Ö©©ë¡Âõ@W¹í«wQe´+ŠöW¸6¾vcî‰®ÝDUQÝQAH©fzÐã&-5> ý:9YôøXaÚê°¬ÝSSÑ®<_X�b#kùµf-që­u-uWX„bïMªœÖØMã©dë„V¶ÐâÌòY±tJŸtê4Ì¡é­€µ­1Kî—nf÷ëiPGíöN[ ´@³­�
 «±´míˆZ®øý½"ïE!¢ÙÚWÕ`çw‚›xmÅö…ƒ 8ÑÚ½/�-cîãýþ¢´ˆ8#Üub#>Ø×Fâãã[�B«õd�ÿ¡AÔ8}˜ƒ€z�«YZ†¥E^D®úa�ú¥!q…�¡XÝ(/�ae6­f/e¦­X=ÀuÝgIÚï˜�¾%—RjÏœ/˜8 Þ§§©x§åt€ZäwjÖ‚Wbz•R"ÕÒk¡b™ÿOÕö­xJŽ)ZhzO_MºÒŸ!Fý¢æ­½}[ªi €MÖ] _…­)"üE‚ÙX�…4©Ò‹]³¹kDe¸¢}ï¯ã]8[�{ÿ…_Tú«b‘€o«Õž¡»jbv[â*Sê…SÒnÖXU‰àV”ašáM)P…"•JeˆE™Þ�®ò©�ÂÿX~8V…Ó•™55«­|¾�T–J×Ñ´Z·A¡£‚(»YµÓx¦žzªøƒ…TEMZTnHZJÎq�D�ÞûjC¢R?‰O×�‚­X[‰|Nz¯EJ¾ZÛ´\+cÇR4›î‹ÿo—’ {†fô½§�Vx½Ú´ŽåoDà½#M‰! ?I>#jÖ¶ADª
"fØ¨ u†EZÞ#ˆVúE·‡dJ¿Z¸|@[m…F©-¿X¾ìï€[�ŒR¼à‚ùövØºÅjÚÅaÙo^%Qºh:‚ëROT6›£SUJ‹Ýè8ÔW‰ŽZÝ-BŽ º=Ø˜eEžd‰Ýu]Õ8ô… `êK�^ÕJŸ}KF"êŽû€NuO÷é¾•ß¨f^*z(ÔQï�xýúf–9V87ùSÅc?oªáx"ü´Æë¹êÚ Mæ/b9éˆ\•b,ï&úî(¶ÑF\á†�ÝÇÓï®¶A¼ÃÍA¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  Å     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #´<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="7" name="MET" directionality="1" translationMW="155540.74" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-5906" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7285" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7888" maxFusedRunOn="7888" detectionMode="exactProteinMatch"><Segment range="7235-7288" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7996-8117" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5952-6163" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9806-10394" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7289-7888" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7888" maxFusedRunOn="7888"><Segment range="6503-7165" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="11356" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="10565-11356" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="11357-11425" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6176-6444" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="9452-9458" color="#ffffff" type="standard"/><Segment range="9459-9476" color="#ffffff" type="standard"/><Segment name="-35" range="9477-9482" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9497-9518" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9404-9420" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9428-9444" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6482" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:51:38">2021.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   a<StrandColors><TopStrand><ColorRange range="5414..5414" colors="red"/></TopStrand></StrandColors>  8o     C  ,¡        ,¡{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØPÿÎ#£~¾ÈïŠŸ �M`‰QäÕ/…ˆY…&Ó¯×­ówöôX¶¹ØM·j¾‚=åçjb©XAGëE
8IÔxˆà]ý*ý‹AãM5OZ›A¨v×`îJ,wç˜aµí¤â2×DãÓ‹«ýÖÙã‡gK+xÙ®ÿtdQ?OH¾"Lþ=Rú¶âT
Û^º‰gÙZë¦¯Ñ¶ö�¬fÒC•qQ‚©Š¨p}/�¡Š§á+Dk§ý¥&/cê5Ö©©ë¡Âõ@W¹í«wQe´+ŠöW¸6¾vcî‰®ÝDUQÝQAH©fzÐã&-5> ý:9YôøXaÚê°¬ÝSSÑ®<_X�b#kùµf-që­u-uWX„bïMªœÖØMã©dë„V¶ÐâÌòY±tJŸtê4Ì¡é­€µ­1Kî—nf÷ëiPGíöN[ ´@³­�
 «±´míˆZ®øý½"ïE!¢ÙÚWÕ`çw‚›xmÅö…ƒ 8ÑÚ½/�-cîãýþ¢´ˆ8#Üub#>Ø×Fâãã[�B«õd�ÿ¡AÔ8}˜ƒ€z�«YZ†¥E^D®úa�ú¥!q…�¡XÝ(/�ae6­f/e¦­X=ÀuÝgIÚï˜�¾%—RjÏœ/˜8 Þ§§©x§åt€ZäwjÖ‚Wbz•R"ÕÒk¡b™ÿOÕö­xJŽ)ZhzO_MºÒŸ!Fý¢æ­½}[ªi €MÖ] _…­)"üE‚ÙX�…4©Ò‹]³¹kDe¸¢}ï¯ã]8[�{ÿ…_Tú«b‘€o«Õž¡»jbv[â*Sê…SÒnÖXU‰àV”ašáM)P…"•JeˆE™Þ�®ò©�ÂÿX~8V…Ó•™55«­|¾�T–J×Ñ´Z·A¡£‚(»YµÓx¦žzªøƒ…TEMZTnHZJÎq�D�ÞûjC¢R?‰O×�‚­X[‰|Nz¯EJ¾ZÛ´\+cÇR4›î‹ÿo—’ {†fô½§�Vx½Ú´ŽåoDà½#M‰! ?I>#jÖ¶ADª
"fØ¨ u†EZÞ#ˆVúE·‡dJ¿Z¸|@[m…F©-¿X¾ìï€[�ŒR¼à‚ùövØºÅjÚÅaÙo^%Qºh:‚ëROT6›£SUJ‹Ýè8ÔW‰ŽZÝ-BŽ º=Ø˜eEžd‰Ýu]Õ8ô… `êK�^ÕJŸ}KF"êŽû€NuO÷é¾•ß¨f^*z(ÔQï�xýúf–9V87ùSÅc?oªáx"ü´Æë¹êÚ Mæ/b9éˆ\•b,ï&úî(¶ÑF\á†�ÝÇÓï®¶A¼ÃÍA¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  Å    Ò      ½®ZTR

BASE      §`  xÁA  ƒ°YãxÔ þµ,9slŠÒ`Su¶fM´MÒ\3mZ[“±1¡mc-`lbL`éZƒh°Hf©`‰Î•”AKÊ�Æ$‘*­­)†T´* ±ÁÐ’ÁV³±†mÓÖlQµJ¥%6™L`
ÉÆÖª�$H›«å£ÑH0)fk“¹ÛÐ¦Ì!-+U„$l6$d¹±•Ø$ÊT‘ƒ1•)kiÃÖÚsjÛ˜K+É°¡[I‚eµØ„PÊ²¨TF’R“rD²Æ`3`0ÚªhU7”¥!5ZÚXÉe¡JÁ¢¥4­Lª¨°¬á–Û 3F3V‰ƒ ÙÊVJ€ÙFMJCÚ€¦ÙÆZ”‰` ÆHl°VA„%›ÍEK&LÛ6•Y3†@lL™6h4¤¬rÛZ9˜0e”M
mäÈ¦´Ö¤ÔH Z‚Ã²aä@h�Ñ2eRn¨�4&6+ˆ±l(I6[ƒ9§k4ˆÈX…"³šÌ¹%mK(™ƒ¥Y ‘¢%lM”˜AÖ6$Ûr–4Ùjm•9’%A5iªLÛ0•”µL¬QkcR€´"¶‰mƒÁHŒTjÐfm³É�JYÚd	M3›*jµ’µ6Ûšâh­9rcƒ¦�¹iÃÙ²i2„­JbMTk°±a³¡Dk³mXmTnlT£`Œ•im@cÌ&ZÃ
ÖGƒ�ÐDV[Ú”Á¤¢¥E%Ð¦¸A„�…”i´6ÒÂ¢r: ÜÒh³­M¬°°Û€Bdmƒ2†H%ÔÖ€´°`­% ‹²­U¦J%ØX5[5€SÐ1†0É’º#•ZFJRP «(ÖÖ6&B [«m hƒ’k©cÐ–´&&l`…¬MZ‚äÌ dŒ [-ÓR› ³A6ÁTm 
L«’VŒ¶‘V
„ÕÈ¤¢…ikL«0e�F&´Mh -l
¶�¨¡6˜¬€­ÁZ¬6 ³5b!eƒéªhâ–*4´Ù€È3 b¬&L¨,A—Ö° V‚2’A%²Q£f[«eB$ÈÄV†µ­*mm6 Æ€	0²m²ÅÊŽ&Œ…ˆiRmÄf-XSkÁT0d@[%Ð [CÇ«”ÁHViP’ÉÀ­¤éÖd‘fC†´T%Œµm˜�ˆ”¥6Â–"54Y3RÆlKÙdM&-Ö¨m*‹i£Ö¤,‰Æ6©Új-"iV«¡Ñ& ÄÖlHk ƒÄ†ÄÆ`•
•Û I0ªX’
-ÍœiÛ ›6k°-ÓÙÖ–†Ò icd9 ’r@c˜)ØYÔfµe	‹)°I"kÂ`ÕRdk³	lÑl£P"KØj6ª¶¬)¤E#`F¬…ÈÖfSÖ`m´YmÀ²	˜ «P–ŒÖÚÒjsÍ4ÛÆl4„±1U´H.@´Ø*¬Õ„m†iê l*Cµˆš4ÛD˜hN%­²�m-u#’9²Ì°’Ú6&FKiœaÑ¸ ZË™.Ò2[-I#p6�­‡2MË¶1$isN�™  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>   ˆ<AlignableSequences trimStringency="Medium"><Sequence name="8508_161" ID="0" sortOrder="0" trimmedRange="0..4190"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #´<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="7" name="MET" directionality="1" translationMW="155541.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-5906" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7285" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7888" maxFusedRunOn="7888" detectionMode="exactProteinMatch"><Segment range="7235-7288" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7996-8117" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5952-6163" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9806-10394" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7289-7888" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7888" maxFusedRunOn="7888"><Segment range="6503-7165" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="11356" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="10565-11356" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="11357-11425" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6176-6444" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="9452-9458" color="#ffffff" type="standard"/><Segment range="9459-9476" color="#ffffff" type="standard"/><Segment name="-35" range="9477-9482" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9497-9518" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9404-9420" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9428-9444" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6482" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:50:32">2021.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  2#     C  ,¢        ,¢{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØPÿÎ#£~¾ÈïŠŸ �M`‰QäÕ/…ˆY…&Ó¯×­ówöôX¶¹ØM·j¾‚=åçjb©XAGëE
8IÔxˆà]ý*ý‹AãM5OZ›A¨v×`îJ,wç˜aµí¤â2×DãÓ‹«ýÖÙã‡gK+xÙ®ÿtdQ?OH¾"Lþ=Rú¶âT
Û^º‰gÙZë¦¯Ñ¶ö�¬fÒC•qQ‚©Š¨p}/�¡Š§á+Dk§ý¥&/cê5Ö©©ë¡Âõ@W¹í«wQe´+ŠöW¸6¾vcî‰®ÝDUQÝQAH©fzÐã&-5> ý:9YôøXaÚê°¬ÝSSÑ®<_X�b#kùµf-që­u-uWX„bïMªœÖØMã©dë„V¶ÐâÌòY±tJŸtê4Ì¡é­€µ­1Kî—nf÷ëiPGíöN[ ´@³­�
 «±´míˆZ®øý½"ïE!¢ÙÚWÕ`çw‚›xmÅö…ƒ 8ÑÚ½/�-cîãýþ¢´ˆ8#Üub#>Ø×Fâãã[�B«õd�ÿ¡AÔ8}˜ƒ€z�«YZ†¥E^D®úa�ú¥!q…�¡XÝ(/�ae6­f/e¦­X=ÀuÝgIÚï˜�¾%—RjÏœ/˜8 Þ§§©x§åt€ZäwjÖ‚Wbz•R"ÕÒk¡b™ÿOÕö­xJŽ)ZhzO_MºÒŸ!Fý¢æ­½}[ªi €MÖ] _…­)"üE‚ÙX�…4©Ò‹]³¹kDe¸¢}ï¯ã]8[�{ÿ…_Tú«b‘€o«Õž¡»jbv[â*Sê…SÒnÖXU‰àV”ašáM)P…"•JeˆE™Þ�®ò©�ÂÿX~8V…Ó•™55«­|¾�T–J×Ñ´Z·A¡£‚(»YµÓx¦žzªøƒ…TEMZTnHZJÎq�D�ÞûjC¢R?‰O×�‚­X[‰|Nz¯EJ¾ZÛ´\+cÇR4›î‹ÿo—’ {†fô½§�Vx½Ú´ŽåoDà½#M‰! ?I>#jÖ¶ADª
"fØ¨ u†EZÞ#ˆVúE·‡dJ¿Z¸|@[m…F©-¿X¾ìï€[�ŒR¼à‚ùövØºÅjÚÅaÙo^%Qºh:‚ëROT6›£SUJ‹Ýè8ÔW‰ŽZÝ-BŽ º=Ø˜eEžd‰Ýu]Õ8ô… `êK�^ÕJŸ}KF"êŽû€NuO÷é¾•ß¨f^*z(ÔQï�xýúf–9V87ùSÅc?oªáx"ü´Æë¹êÚ Mæ/b9éˆ\•b,ï&úî(¶ÑF\á†�ÝÇÓï®¶@o0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{  Å     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #š<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="7" name="MET" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-5906" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7286" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7889" maxFusedRunOn="7889" detectionMode="exactProteinMatch"><Segment range="7236-7289" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7997-8118" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5953-6164" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9807-10395" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7290-7889" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7889" maxFusedRunOn="7889"><Segment range="6504-7166" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="11357" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="10566-11357" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="11358-11426" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6177-6445" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="9453-9459" color="#ffffff" type="standard"/><Segment range="9460-9477" color="#ffffff" type="standard"/><Segment name="-35" range="9478-9483" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9498-9519" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9405-9421" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9429-9445" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6474-6483" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:49:57">2021.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  2;     =  ,«         ,«{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØPÿÎ#£~¾ÈïŠŸ �M`‰QäÕ/…ˆY…&Ó¯×­ówöôX¶¹ØM·j¾‚=åçjb©XAGëE
8IÔxˆà]ý*ý‹AãM5OZ›A¨v×`îJ,wç˜aµí¤â2×DãÓ‹«ýÖÙã‡gK+xÙ®ÿtdQ?OH¾"Lþ=Rú¶âT
Û^º‰gÙZë¦¯Ñ¶ö�¬fÒC•qQ‚©Š¨p}/�¡Š§á+Dk§ý¥&/cê5Ö©©ë¡Âõ@W¹í«wQe´+ŠöW¸6¾vcî‰®ÝDUQÝQAH©fzÐã&-5> ý:9YôøXaÚê°¬ÝSSÑ®<_X�b#kùµf-që­u-uWX„bïMªœÖØMã©dë„V¶ÐâÌòY±tJŸtê4Ì¡é­€µ­1Kî—nf÷ëiPGíöN[ ´@³­�
 «±´míˆZ®øý½"ïE!¢ÙÚWÕ`çw‚›xmÅö…ƒ 8ÑÚ½/�-cîãýþ¢´ˆ8#Üub#>Ø×Fâãã[�B«õd�ÿ¡AÔ8}˜ƒ€z�«YZ†¥E^D®úa�ú¥!q…�¡XÝ(/�ae6­f/e¦­X=ÀuÝgIÚï˜�¾%—RjÏœ/˜8 Þ§§©x§åt€ZäwjÖ‚Wbz•R"ÕÒk¡b™ÿOÕö­xJŽ)ZhzO_MºÒŸ!Fý¢æ­½}[ªi €MÖ] _…­)"üE‚ÙX�…4©Ò‹]³¹kDe¸¢}ï¯ã]8[�{ÿ…_Tú«b‘€o«Õž¡»jbv[â*Sê…SÒnÖXU‰àV”ašáM)P…"•JeˆE™Þ�®ò©�ÂÿX~8V…Ó•™55«­|¾�T–J×Ñ´Z·A¡£‚(»YµÓx¦žzªøƒ…TEMZTnHZJÎq�D�ÞûjC¢R?‰O×�‚­X[‰|Nz¯EJ¾ZÛ´\+cÇR4›î‹ÿo—’ {†fô½§�Vx½Ú´ŽåoDà½#M‰! ?I>#jÖ¶ADª
"fØ¨ u†EZÞ#ˆVúE·‡dJ¿Z¸|@[m…F©-¿X¾ìï€[�ŒR¼à‚ùövØºÅjÚÅaÙo^%Qºh:‚ëROT6›£SUJ‹Ýè8ÔW‰ŽZÝ-BŽ º=Ø˜eEžd‰Ýu]Õ8ô… `êK�^ÕJŸ}KF"êŽû€NuO÷é¾•ß¨f^*z(ÔQï�xýúf–9V87ùSÅc?oªáx"ü´Æë¹êÚ Mæ/b9éˆ\•b,ï&úî(¶ÑF\á†�ÝÇÓï®¶Ï�Ì<ÔŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #¸<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7295" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7898" maxFusedRunOn="7898" detectionMode="exactProteinMatch"><Segment range="7245-7298" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8006-8127" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5962-6173" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9816-10404" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7299-7898" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7898" maxFusedRunOn="7898"><Segment range="6513-7175" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="11366" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="10575-11366" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="11367-11435" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ORF #12" directionality="1" translationMW="155954.22" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-5915" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6186-6454" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="9462-9468" color="#ffffff" type="standard"/><Segment range="9469-9486" color="#ffffff" type="standard"/><Segment name="-35" range="9487-9492" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9507-9528" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9414-9430" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9438-9454" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6483-6492" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:49:23">2021.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  %Â     <  ,¨         ,¨{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬÷ØPÿÎ#£~¾ÈïŠŸ �M`‰QäÕ/…ˆY…&Ó¯×­ówöôX¶¹ØM·j¾‚=åçjb©XAGëE
8IÔxˆà]ý*ý‹AãM5OZ›A¨v×`îJ,wç˜aµí¤â2×DãÓ‹«ýÖÙã‡gK+xÙ®ÿtdQ?OH¾"Lþ=Rú¶âT
Û^º‰gÙZë¦¯Ñ¶ö�¬fÒC•qQ‚©Š¨p}/�¡Š§á+Dk§ý¥&/cê5Ö©©ë¡Âõ@W¹í«wQe´+ŠöW¸6¾vcî‰®ÝDUQÝQAH©fzÐã&-5> ý:9YôøXaÚê°¬ÝSSÑ®<_X�b#kùµf-që­u-uWX„bïMªœÖØMã©dë„V¶ÐâÌòY±tJŸtê4Ì¡é­€µ­1Kî—nf÷ëiPGíöN[ ´@³­�
 «±´míˆZ®øý½"ïE!¢ÙÚWÕ`çw‚›xmÅö…ƒ 8ÑÚ½/�-cîãýþ¢´ˆ8#Üub#>Ø×Fâãã[�B«õd�ÿ¡AÔ8}˜ƒ€z�«YZ†¥E^D®úa�ú¥!q…�¡XÝ(/�ae6­f/e¦­X=ÀuÝgIÚï˜�¾%—RjÏœ/˜8 Þ§§©x§åt€ZäwjÖ‚Wbz•R"ÕÒk¡b™ÿOÕö­xJŽ)ZhzO_MºÒŸ!Fý¢æ­½}[ªi €MÖ] _…­)"üE‚ÙX�…4©Ò‹]³¹kDe¸¢}ï¯ã]8[�{ÿ…_Tú«b‘€o«Õž¡»jbv[â*Sê…SÒnÖXU‰àV”ašáM)P…"•JeˆE™Þ�®ò©�ÂÿX~8V…Ó•™55«­|¾�T–J×Ñ´Z·A¡£‚(»YµÓx¦žzªøƒ…TEMZTnHZJÎq�D�ÞûjC¢R?‰O×�‚­X[‰|Nz¯EJ¾ZÛ´\+cÇR4›î‹ÿo—’ {†fô½§�Vx½Ú´ŽåoDà½#M‰! ?I>#jÖ¶ADª
"fØ¨ u†EZÞ#ˆVúE·‡dJ¿Z¸|@[m…F©-¿X¾ìï€[�ŒR¼à‚ùövØºÅjÚÅaÙo^%Qºh:‚ëROT6›£SUJ‹Ýè8ÔW‰ŽZÝ-BŽ º=Ø˜eEžd‰Ýu]Õ8ô… `êK�^ÕJŸ}KF"êŽû€NuO÷é¾•ß¨f^*z(ÔQï�xýúf–9V87ùSÅc?oªáx"ü´Æë¹êÚ Mæ/b9éˆ\•b,ï&úî(¶ÑF\á†�ÝÇÓï®¶Ïó5ã;Â?- 4LÝ³÷KüE( h\ùBÀ%xHbÈžóê¯Ä EòfR4’Ï!rº«5À¨óÑwNëÍ»¾·3óÏçá=½Ï
Ì®<ûóâûáxËÏ.BR¥NÐ±ê/ïèçä{Oïs¨øŽŽ×¹Ë¨«*¸®3ÊtoSˆ|3ç9÷Ø1ÄL>?=�<Û|7ø\2¬N0DÿÔÐ<Ø}uQ7Õþ­?çã„÷!€ç¹÷¬7çý0çëãsÛ]pÃ�õßÍ±œAÃŽ7!:ÓŸ9Ä<<Èl1ëP‚|:ÿtÈn·ÇQ¶óMsß Møßör8 4ë?7ëOŒÇ9çN²=ØÞµ=½ÿM¾4\ ÿb³ëÏ2�Ó]ñ8²ŸMÏ­GÿlúÏFüÎÒËÖ¸òóÁü;ßìÄRë—croÃù‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�…m~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰´¥è©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.¡íXbÖ’æÑNn¿¯ Goâ¥š•ÓH¯Õà+¦oèî_B�¯‹íÐ\RþTWH4ô*OðZ¾ª�?h&ªÿ&ÿô"ãCµDÓL­TÆü�~¿Èü/ós�ÀÀ3ÁðLü;8ãÿ“ –�¿€  ‹øfæ]EY™™e)·%$]…eÖe¦É„¥n•KrUmŒ¶¨{s)’µ[Hw§Í¡ÔÜûp½L1á¾V7Lpk3š‘Q5šµ62Ö©Ín®@¥¹8ÛA¶l°.ª¼;KlýNæà6ÀQOÈú¯ÌB…0Ø£Äxã�¡Êsr§Øa¬ƒØÜú—^++Cß€gÒ²Ì:¯t¯OÅ3m5ü]gÃ<�#Â#F–0Œ3€fœ´Ä	¾<ÑX�ŸòŸÃ3 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Ô<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="Feature 12" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1728-5912" color="#a6acb3" type="standard"/></Feature><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1710-5926" color="#ffff99" type="standard"/></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="11363" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="10572-11363" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="11364-11432" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7895" maxFusedRunOn="7895"><Segment range="6510-7172" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7296-7895" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9813-10401" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5959-6170" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8003-8124" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7292" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7895" maxFusedRunOn="7895" detectionMode="exactProteinMatch"><Segment range="7242-7295" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:15:45">2021.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  %Ä     >  ,®         ,®{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¯±}…üâ:7àÛìŽø©úÑÖ H•ÞMRñX…˜Rm:ýzß0WoE‹k�„Ûv«è#Þ^v¦*•„~´P£„�G€øŽßÑ¢¯Ø´4ÓTõ©´Š�Q—mvä¢Ç~y†^ÚN Ó-t@~=1Øº¿Ýmž8vt°B·�šï÷FEôô‹áâ „ÏàÕ/«n%Að­µë¨�Ö}•®ºjýohÚÆm!9PW*˜ª‡ÒùÚª~´FºÚRbö>¡S]jšžº/T{‘ŽÑê·u[B¸¯e{�£kç`V>àHšíÑTEU½ÕŠ–g­2bÓSàÓ£‘Õ�OO…†®« ŠÍÕ5=ãÅõ�±¸ö"6¿›Vb×º×Q²×UuˆF.ôÚ©Áým„Þ:–AÞ¸Ekm!LÏ!…›D©÷N£LÊšØZÓ¾évæo~¶•~ßdåºÛD:ØÐ¢
»Aö×þØ…ªï�ïÛÒ.ôR M�¥}Vwx)·€QFÜQ¿hX:�«ÒøÒÖ>î?ßê+Hƒ‚=ÇV"1°Acá0�ítn.>5¹Ô*¿VHßúC‡Ùˆ8¨°Ae•¨jTUäJï V8ß¡êR7YÚ�Ò‚øöSjÖböZjÕƒÜ]Öt�®ùˆâYu&¬ñYÂùƒ‚êzz—�Z~WH®Gv­h Uv'©U"-]&º)Ÿôý_j×„¨â•¡V‡¤õñÔÛ­)òoÚ.aZÛ×Õº¦’ÝeÐŠUøZÒ’/ÄX-•ˆØSAAz� ØµÛ;–´F[Š'Þúþ5Ó…¸×¿øUõOª¶)ú½Yê¶¦'e¾"¥>¨U=!†í`5…Xži@V¡þÒ•R)T VQ8„Y�éÚáO*˜Ü/õ‡ã…h]19Y‘ SSZº×ËèId­}E«t8"‹µ›]7Šiç *¯ˆ8UDTÕ¥Fà4…¤¬çÔIÝï¶ d:%#ñH”ýyÑX*Õ…¸‘çÄçªôQ´«å­»EÂ¶<u#I¾áÑØ¿öùy ¸foKÚxÕg‹Ý¡ûHîVôNÐÒ4Ø’ô“á¢6­aKd@Ú ¢&m€zŠX`ÔU­â8…o¡t[xvA«õ«‡Ä¶ØTj’Ûõ‹îÎø¸øÅ!@»Î/Ÿga]�¬V­¬Ví–õâU¦ƒ¨.µ$õCaYº55T¨½Ñƒ�Ex˜àaU­ÒÔ(áª£Ñ]‰†TYæH�×UÐ�S�HR T¸ÕíT©÷Ô´b ¾ Èï¸á‡Qÿ~›áÉ]ú†eâ§¢�@Eù×ñg�Ñÿ¦aIc•cƒ•<V3öú®‚/Á›Ln»ž­ Þbö!sž˜…ÉV"Îòo®â‹meÎaÜ}>úàÛ`Ìðo0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Ô<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="Feature 12" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1728-5918" color="#a6acb3" type="standard"/></Feature><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1710-5932" color="#ffff99" type="standard"/></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="11369" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="10578-11369" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="11370-11438" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7901" maxFusedRunOn="7901"><Segment range="6516-7178" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7302-7901" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9819-10407" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5965-6176" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8009-8130" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7298" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7901" maxFusedRunOn="7901" detectionMode="exactProteinMatch"><Segment range="7248-7301" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:15:18">2021.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   è      )  Z         Z{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬Z•°o0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1710-1752" color="#ffff99" type="standard"/></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7189" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6398-7189" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7190-7258" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3721" maxFusedRunOn="3721"><Segment range="2336-2998" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3122-3721" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5639-6227" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1785-1996" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3118" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3721" maxFusedRunOn="3721" detectionMode="exactProteinMatch"><Segment range="3068-3121" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ª<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:15:0">2021.2.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  $ý7zXZ  æÖ´F !   t/å£á!gä] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷¿Üµ¡&õý{ÍD¹ÜŠcºa§k%ýP1TúbÚR‘T›iCKÞ[8øý~]?¿Ç80ìa�äÀ—yÐ�F”z?;n1uA‡TÓ:lCÒóùùà…:‚[aXØÜ#�#êñ×:+UÔ Ôf=ôx%ŒcT?Ô+)7h‚¹f�:sî‡›qžìßûÜV|”�HXP¥O…8]Ý€×¥"ééÂÃ4†ßñ¢Wƒò¨¨·ò¹GY0¶:=ŽHg¦6ü¬ÒN@2D›<æÄ³Dáå:�¦:¹Kn«0õ¥º
ä¯Ú¤^=Œ™U:MH´ÞöšˆõGæ$�¢—Ä°ýJ¦-ô€‚àgŸ÷56�§ª‹Ò…�­Öô�8³•˜øƒO.¢q[5x/ªÛm…š`ùéÞÐÜåÄ]¨½tž¬ð±IuêüÀ�àÝ¥2_˜’�<YÜ=§…[H5È-.Ðô�=ž°-ÉYƒ$ÃwlóÑ§Ÿ&2Ë½1è}ÓÖ³Næ`iñéÒ™\·çÀ«:„õãNLh£ÊïÊs`Ås95œƒãà±ÓkÔ·9��Z:­%"¼ñÔa¦nGÈ�ù¸MÝ´Á~ª°‚†é}×ñÍZqÝ8¿hc¢Ú ÖcÖX@“ûÎ·*ô‚XóÐ—¢:ÿ+-IZ½u½Ð\}'îø@J�t™Eý--¬Ù#jV6a�ä¨]^T’šOjn�|öM{©#¤"«`˜¤Þq…v'à×-ëd‚&JsÝX¦É°T|v<o­”„:ôd/²<ð…üÓÓJ’›Lš4?eG½¥dÏëÁÍ9ã«%~q©$vƒº�(ž,XŸ½Ž÷±�¦ÞÈ™PÄÄUû:nÓéRf/Úç×c	OÎ_XYô--¶®MØ€¾ŸšáS¢ñ]ìû* TÌkëÁ©ê}|¹Ëz­±k5``�Œã·î4U¡´*m�!}Ïv¿û;ð!7Ì\c¿­&ùBæsD
:ÃÚÑ´AÁ»:�!€`mÆ´îI"ÎW¼	¬’øµ¶1øoãˆú .|”Ýz«Ú…Ç™Ýái¼Q
‘ë*(ì^`þW²•ä½"Áññ]ÖéÞ4ùÓx…¾A�&–	¯…ð^^é<Øš“”Ó*O×'QãCêàÇC¿ßŠ[µpk“�F¼É÷«–èO+ Æ“~øöÌ`t�sÊD…P‡6Ê\¾3�×LÃ}å¼»¢à#Á¯BŸµ;½§¹ï¤Ig£€sË§m¢-ÔyC¾ä'[yy�ô¤-ï–›_*ô¢5q³3¨;ƒl�êU—p£�I¬òuÿ§
"=l³5ì<PÞÿÊóŸŠ/Ÿ*´_Õ9
#èA~Q´³êÐY–›#ãî„¿Q�î4$$<2Ï��#²·º½ZÄ*Ñ½ˆ`Ûƒ]qbÎÏÙ`CªâïÅ5…FˆmÓã–ôƒhÍÃ|7Ý\~¿	žÙîa.fñÌ°‡­óµyz¡—ÜQÚ²XÏÿK9ÑÎÍ÷ [_;¦éWßŠÿÛ�ë“Õƒ†Ç~rS‹�lí7ó›9øÛUov`gC^3£«ä$­xÓÛ�–øp]oæ�RB”Îö€c�PzÙd/ò`i‘­�ÒE®Z?ZÍå"0S´†eÍ¼X¥]9@0j¿ÿ¯JD9ñ-#çÝLF¶ÃÃ©Ù×HAyxxP(´éœQ>jonHƒÙ3úp#0ívCA-7®ºø•»XWø+ëµ'0ŒJ[Aß TÆ{p×ÄrSü-¬3àø€¾ëqÛFÕ˜óÛ2lì+”1G,\'mäy6(…¹…·µ�tkyÄÝcÔ³å»
­‚C°Wè­`|ízyÛfG!0øv;âzÍfL ™èp¶Ý£57‰™ÖŒ$5A·fæ¢”…êZ¬›kÒà<õ1È>9<«[©J
;25²|Mï¼‡›»6œ¥Å$F¤m¡Y\m¡pqîQ%í¾>B­<<ª%Õ��ÄLºÓJêe•â�2hEºî5ú©®¡²¨u-ÐÞ´Ô®°•¥týª0¨ïŒL N{Ú™¶÷‹°YSÝÜŸ.ê;.„ëw¼ìv¾ú»TBª©çÀ%Øl0²Í)ëþëå÷“‡U‘­JSîê±w­Y�ýeIã¥l€Ô†ø˜eû2&úJ^ºkæ…�£„ ÝDú”¥y*	†ç—½;ÅÔSI½{<ÇÆWvA�i-Æ<JÌiÇþBÌ/cCI€eïFÃÐ§š{ÕÒèdü+¥�?Œ˜Ì©ãŒ¡n»9ýqPäáO¥s[!Ÿ�ê2|Î Pn´õê?z¦È|+Ñƒ7!º¿h(ý(KÝo?‹	ÿÎ}���sÒî!´?¨¯dú”ÿÍµ#•GVZ^pg| “”Þ=y¤7
ÕÂË�I
/ ¨;§«‰Ü@C¿LOß�E=Ñ§"~‘ù£©ÖZKX‘ùúAírÎ÷ñg¦új^\â÷ë/î †=ØFM‚®ß$›¾.*‡ú;¹-ÖÈ=¨Ù_ÑjÔm‚ŸÑ¦�‘·pý…Íœï¯=\uBú“*ê<›Í{Ô9@§lšA’;Ò\w<†#˜4äj?$¡Ñ–¬!¢1•©žg5'Û÷æ®ÛeK…Ä|k·D	D§É³SLïæçdÏƒÍ?¿¤ÊU;7¬”Ôx”g½Pï@K×åy³~0f#eÐk;œè‰ntB”Pæ{˜kBhn™Ÿâ“‹@òùz$%µ&†‹9æÐVo6ÂÀ‚^ýrVF_§‡ÔŒà¾�õêáÅ-Äy”NX²Jò¾Óº–SÆyæ��‘nc}	fàG_Éª©ÎÎ�E+ÀoËòü„ºûµÒ �]°àÞÿBøÕ.zØtB(m÷|“ì–n‹Ö·ª9©Hü2:4\"výHÜ«‰Ïm¼Û¶Ê8¡U
L¡^� :(}«ÁL‚�Ç§¬i&�Z³u�áTÀ™'³u§ÊËÍBè‚×7È0ùB3ƒ×&+–h¾©.!ßÇÐ­‘ÄAN†¶Ï<¬b’+û‘‰ CaMœt¹dN¡îI`®dæw�©0ÈPMóëä–úÇ<™?wílÐø	ÈÉrQ­–=ÔˆŠ09øn1o34Ý@Aí÷gE—4ÎÐ¡-õ¬ÐA·¾ð¬TÉr3 ¡�Nà¦ñÍ„ðœ›ý�˜B(CÍQê­ëöZ‘‰c¥º0ãŠ‰¡³.»Á*@;²¢ô� F9b€æ@Zó¯�µ\]œõw±™•VÁú-îñi y²-¹áh\æ8ÓÕ0á‰M®÷ïAWCtíÿb-z»Â#Ä!�§†Ã�7ëÃƒ9¨òÔ,E:]pI¶«„Og€õÉš�ËMëKƒµ�ù¼Æ­KVe9&6ƒÌ´¯W†išS³šÔ°>óÀU˜„'7Rû'j›Ï´ƒj•<¡ƒ%XßÆžƒ%·¨R1×–îìƒÈFWš–R‡2sž	š×W÷Hê³8Uõ­‘ya‘,W�ã:téÛ‰å�Þ¢WƒÂ³™&6ŸP!*=à€7ˆvoñÊV�/zïî† «&UÂ‘k°U!Ë?ÎT„‡m>TéIˆÔ‡¯{UV1¨OM’DÄ‚%c¯à.0ú€	?¥=˜OA…éx÷ª—m-×w—a¼¿$y¯D0üøànŠ]¨"ƒkÖï´7Êp6¢fbDÒCwïçî]ZÏ†ùêí„Ë"¿Ã0]ÔdÁ` E²ƒ;;P‹êU¹-bØÒ®0Ë¤1=ÓDV—.®±¡wš½p*FÂ1y4-¤·þnð?§�~9öÜÕ®®¬j,ÆŠðBl0Þ¶�, á1iŒº|ôô¡ˆ½$é®'ßªF÷#Å(2ç×£ÎùíìgÆÊl‰Y'«Â›ÊþÅÖ¥bžZyÃ½AüDkÛ*ÇÝ�Ý×3�áŽÛ9ù|€f½1®u[›Yœ6iA)ÃpÖM\ûƒ:Ö¥ÀfÞXO–cO 3hoLî‡Ë>^p±ffÇo™<ê ¬Mc°ÁHÿËžà©‚kh@ãs9ý)!ñˆÃæK>¡ë×”ðKÐ1”TpáDgkÞÔÑT
çt¸�ÿ°+–Ý<™gG�L \×Ìÿ´¼Ã_‘ò$ø…nJÆç5KçßÊ�.ï=ãÛ «=gÈz£Î­Rv}.ëÇŒ£O@€ß,B¥Æ`.€ûÚÑ¨–=ÔA&×ÆêÍHTèšçîqî*Ç`2Õ™¯Y�îñB¤ì1#_NËµé½˜ÕY>*çÈ«§Ò_mÂ™!†¿‚ur2òc›¶ª¹ª§Jˆf´«(Ù¬eðX/òS˜#I¶­ïHacPÛ12¼D$ÂÇÐÃÇw{ö>D/¿�fgïB²O›®¾7ª’ECW"Œ]ÞÍQ5¤ÓH€Þ< pUaã-ÎÜQIîð.Û#, †<J½ty¦àL¬!ZQø—ò,/›&ùÏÅŸ²xeKG,Šg,¸[.¾ÞŸšƒ3¼¾ÞÎôø–(!Ì8³z8êŸm0æÚèR¶a·/ÿ²ü–<ÿk„ÛñÎ)Ÿ¥’Ó�2ƒG‘Î�ŠÝ+ÇÀˆÏ4&@1e¹,ÑÖõÈ©$ÉÆ˜Ö0Œø;{y™|ÜjˆµDRSç`$~õ`§Ç6Ë?üKÚbô ¥ÜùÈ+×Û',’#L�sƒb¹üÞ¯þÄ3ÀeG@��3¬z:C`f[FQ~wÁvÄS ”Ý1Iùj°0kã){JÐ–]>"^\6-íü2Y‰å&¼“©ýÑŠ�V¬Œ~<"€yúe±^PBTÚ†§Ÿ}4›âáp`Ùk7>Rá®[s�PQ£&(P§qNä¥x@‚Æ/(„yé._nUOHL´HÍ±{Ö+„ÿhe­5È¾Á;oJ»¿s%êuE©‚¨ú¿Ã†Ý�Ú19W‡s±è6�³”ËüÄ]ô_R÷nIEoû_§25+8•fE¸‰;Û””_2ÃÕáò™g¢E]q�Q4÷)²sÖ¸Ârƒ ²à¡QhÚJ‚ZÕ¤'JP?ú–îƒø¾,j7"}[Æ0�­@6ûÙ»IM8ME“Â^‹úcƒÅaÉó’‘”áô×Ù®Öíeø•kÔr?Q)HZ ,C¶Ìñ¯w\ÄÃSÊ€“-�(ä	mÇë1ú5QGbœ0�&ýËIC¾A˜ü4eÒR¢ea(q*‚â�zÑôÕPE}th¦ðÌ?íIm]}ù¨š‰¸û§•   ³ˆaÖ”»« € èÂ ü¨ù±Ägû    YZ  Æ      ½®ZTR

BASE      §`  xÁA  ƒ°YãxÔ þµ,9slŠÒ`Su¶fM´MÒ\3mZ[“±1¡mc-`lbL`éZƒh°Hf©`‰Î•”AKÊ�Æ$‘*­­)†T´* ±ÁÐ’ÁV³±†mÓÖlQµJ¥%6™L`
ÉÆÖª�$H›«å£ÑH0)fk“¹ÛÐ¦Ì!-+U„$l6$d¹±•Ø$ÊT‘ƒ1•)kiÃÖÚsjÛ˜K+É°¡[I‚eµØ„PÊ²¨TF’R“rD²Æ`3`0ÚªhU7”¥!5ZÚXÉe¡JÁ¢¥4­Lª¨°¬á–Û 3F3V‰ƒ ÙÊVJ€ÙFMJCÚ€¦ÙÆZ”‰` ÆHl°VA„%›ÍEK&LÛ6•Y3†@lL™6h4¤¬rÛZ9˜0e”M
mäÈ¦´Ö¤ÔH Z‚Ã²aä@h�Ñ2eRn¨�4&6+ˆ±l(I6[ƒ9§k4ˆÈX…"³šÌ¹%mK(™ƒ¥Y ‘¢%lM”˜AÖ6$Ûr–4Ùjm•9’%A5iªLÛ0•”µL¬QkcR€´"¶‰mƒÁHŒTjÐfm³É�JYÚd	M3›*jµ’µ6Ûšâh­9rcƒ¦�¹iÃÙ²i2„­JbMTk°±a³¡Dk³mXmTnlT£`Œ•im@cÌ&ZÃ
ÖGƒ�ÐDV[Ú”Á¤¢¥E%Ð¦¸A„�…”i´6ÒÂ¢r: ÜÒh³­M¬°°Û€Bdmƒ2†H%ÔÖ€´°`­% ‹²­U¦J%ØX5[5€SÐ1†0É’º#•ZFJRP «(ÖÖ6&B [«m hƒ’k©cÐ–´&&l`…¬MZ‚äÌ dŒ [-ÓR› ³A6ÁTm 
L«’VŒ¶‘V
„ÕÈ¤¢…ikL«0e�F&´Mh -l
¶�¨¡6˜¬€­ÁZ¬6 ³5b!eƒéªhâ–*4´Ù€È3 b¬&L¨,A—Ö° V‚2’A%²Q£f[«eB$ÈÄV†µ­*mm6 Æ€	0²m²ÅÊŽ&Œ…ˆiRmÄf-XSkÁT0d@[%Ð [CÇ«”ÁHViP’ÉÀ­¤éÖd‘fC†´T%Œµm˜�ˆ”¥6Â–"54Y3RÆlKÙdM&-Ö¨m*‹i£Ö¤,‰Æ6©Új-"iV«¡Ñ& ÄÖlHk ƒÄ†ÄÆ`•
•Û I0ªX’
-ÍœiÛ ›6k°-ÓÙÖ–†Ò icd9 ’r@c˜)ØYÔfµe	‹)°I"kÂ`ÕRdk³	lÑl£P"KØj6ª¶¬)¤E#`F¬…ÈÖfSÖ`m´YmÀ²	˜ «P–ŒÖÚÒjsÍ4ÛÆl4„±1U´H.@´Ø*¬Õ„m†iê l*Cµˆš4ÛD˜hN%­²�m-u#’9²Ì°’Ú6&FKiœaÑ¸ ZË™.Ò2[-I#p6�­‡2MË¶1$isN�™   �<AlignableSequences trimStringency="Medium"><Sequence name="Copied Sequence" ID="0" sortOrder="0" trimmedRange="6..4182"/></AlignableSequences>  l‹     ÿ BC N srôe”```pðpáó³2Ô3ãö·²*Í+Î/*IMábÊqqQjZjQj^r*ÃB W*2 6   ‹     ÿ BC  í•;²,5†P#•…HÕ�B¶`—³
6b—oŠbi—¯oFL:
Î™™vÛÒÿòßöõ½þùåëëÇoèO|þó×¯ÿÔï_ñ÷×Ÿø*þçÓ†‡õ6"FU–G‹³ÆÉu5k�Öú�š}-imèÒÎÓ¬ÛêÆ‹û´<!yôyºxøqùMµ>ÌýÞ¸³B·n¿»îëSz\ïmUö¥ÒsŽ\£ºj4«{‚¦–�æ2Ü®ùlkÝ)ÍÄ�x�³W�î±Óz™ë±Mû£=<í³GE{ÔVÞ•cÊA¾}=}^ÏavN¸÷Ý[¾V”VÕêcDÍŒ˜×Om™ú¬Ã7ïjCv˜Ø¹¹i²³Þa§ƒA5™)­ËC“ó^[÷ö§©ßvTcæÎ»¶Û®ã·¤¹
ë/G”Îã2»d6§é½îÞdÀÍu|ódZù�íÕ¥â¼0�6‘Þ†Œ�éEÀKöÎÓF=œÕ¼1Ùˆ–•MÅ ÕGvÐSæ¹Ï\ëÞdY³SÑÕmÚc9`80Í…}ä¡O@nöB-âÈ ×9>ì^� ÝxsúTÖÒHÒEÉã)„c$¡.÷q!y¬ÒkÇær;í‘çBóÞÇQÉÔG}�mYS‚×“Çú©uóEçÕä='§¢¡ho_½•U!Eµ-!Rc»çDS9{“~´ƒ™ßn“Ýs4µû²6ÚyñŠPhºˆdæ+äî"�¡%T{h‰óé«Ë-UP@€×Y2Ûä§ìaönÒóN»ïû5Ý~·tÑÝ�¿¬¶–ëéÝÈªùdhHlh»ó¼ŒÈn~ÏË‡oC‹b3tvWT£'9É¢8ÀÆ#†¸ÒÞ;ç†¡œb;¶øšÝÎîþnŠ[’×»@ZA¹²SÔ5Å-!ú.Ã¹wn.‘Ûs×§¡¬ahîêS@VgÓø± zË»o+ÎBh[qtME„¼ WÑáà€ÈäŠODœWy´\íÉ”×?ˆ`Õ=¤N¹6^Ï!‘`H\$ž©‹;æP½eù&Ïª¨ÔÀš‚ÎÑâ�äéÖ9Tû¾æ+³Ò@®í-ô‹‡^»²b<øv k°C1 ¡íi)f­34Â·Ä"ÙAð…Ü@¥îÙ
:îJ7"¦y®}u#Ã{‰_óÝÔ’ “>Q7;ŒýØ‚SÁoõ�r‘J`�%ð“¤„*Àf5ÝÑl¾^0_d„¤w%1º·òEä—"ýxJ6?³¿\¤ËÎÈPÓ¹¶°Û¨mð:½.›á‹”±7/ÀU–o’~’…SÙË^é%p RLÝã,IJÏ&ôø×ƒ('d¡§ßÛPiƒ%¹›æËzÈ‘¹’«k!n2kàÆ†!ãÍ¸‰Ë�n•0ç÷}'î;W¢–õFª@ô\DÂ¬x›Ëe\I¨›.É‚‰ÚßA’«…3–uH1Ý{AIˆž¹1ç«ÆƒYlD2È›uý½c“ñ^¯ÌÀŽÀOI‡8u‰j^`Wtšuzì@¢À.4D#P6žZRI˜åôàÚ	æ|^æ.²DR'&Š�›*êõÜ÷§äár’³È1°|6V:¨�”–¾õmªÚ"¼•ë+xÈ�HêñýÛ·O}êSŸúÔ§>õ©O}êSŸúÔ§>õ©ÿWÿ¢¹ÉáÁ  ‹     ÿ BC             !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,¸<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="0" name="MET" directionality="1" translationMW="155540.74" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1734-5906" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVLEHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSALGAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLDAQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAMNKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEFAVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVACQHRSNSEIICCTTPSLQQLNLQLPLKTKAFFMLDGILSKYFDLIYVHNPVFKPFEKPVMISMGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHLHSEAVLCTVPNDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFTGLIAGVVSISTALLLLLGFFLWLKKRKQIKDLGSELVRYDARVHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNSSQNGSCRQVQYPLTDMSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDGKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMKYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARNMYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAEMRPSFSELVSRISAIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSEDNADDEVDTRPASFWETS*"/></Q></Feature><Feature recentID="1" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7285" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7888" maxFusedRunOn="7888" detectionMode="exactProteinMatch"><Segment range="7235-7288" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EGRGSLLTCGDVEENPGP"/></Q></Feature><Feature recentID="2" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7996-8117" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5952-6163" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9806-10394" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7289-7888" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="9" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7888" maxFusedRunOn="7888"><Segment range="6503-7165" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="11356" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="10565-11356" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="11357-11425" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="11" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6176-6444" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="9452-9458" color="#ffffff" type="standard"/><Segment range="9459-9476" color="#ffffff" type="standard"/><Segment name="-35" range="9477-9482" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9497-9518" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9404-9420" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9428-9444" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6473-6482" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ¼<Notes>
<UUID>e29dfd8b-4f88-486b-b360-1edcd70f5ca3</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac-CMV-MET(human)-D1228N-EF1a-CopGFP-T2A-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="4:44:14">2025.9.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO  P              E              ÿÿÿÿYô? P                                                                                                                                                              a<StrandColors><TopStrand><ColorRange range="5414..5414" colors="red"/></TopStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
